货号：04-1471

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Chk2 (Thr68) Antibody, clone E126, Rabbit | 04-1471 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: The antibody detects Chk2 phosphorylated on Threonine 68. View All» Molecular Weight: ~62 kDa View All» Epitope: Phosphorylated Thr68 View All» Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human Chk2. View All» Modifications: Phosphorylation View All» Clone: E126 View All» Isotype: IgG View All» Background Information: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Application Notes: Immunocytochemistry: A 1:250–1:500 dilution of a previous lot was used in IC.Immunoprecipitation: A 1:50 dilution of a previous lot was used in IP.Immunohistochemistry(Paraffin): A 1:100–1:250 dilution of a previous lot was used in IH(P). View All» Control: Untreated and doxorubicin-treated HEK293 cell lysate. View All» Quality Assurance: Evaluated by western blot on untreated and doxorubicin-treated HEK293 cell lysate.Western Blot Analysis: 1:500-1:1000 dilution of this antibody detected phospho-Chk2(Thr68) in untreated and doxorubicin-treated HEK293 cell lysate. View All» Presentation: Rabbit monoclonal IgG in buffer containing 50mM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O96017 View All» Entrez Gene Number: NP_001005735.1 View All» Gene Symbol:

• CDS1

• CHK2

• Cds1

• EC 2.7.11.1

• HuCds1

• LFS2

• OTTHUMP00000028871

• PP1425

• RAD53

• bA444G7
  View All» Alternate Names:
  • CHK2 (checkpoint, S.pombe) homolog

  • CHK2 checkpoint homolog (S. pombe)

  • checkpoint-like protein CHK2

  • protein kinase CHK2

  • serine/threonine-protein kinase CHK2
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunohistochemistry (Paraffin)

    • Immunocytochemistry

    • Immunoprecipitation
      View All» Entrez Gene Summary: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains aforkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase,preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq]. View All» UniProt Summary: FUNCTION: Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium.ENZYME REGULATION: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation. SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note= Isoform 10 is present throughout the cell. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 9: Nucleus. Isoform 12: Nucleus.TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.INVOLVEMENT IN DISEASE: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500]. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. Contains 1 FHA domain. Contains 1 protein kinase domain. View All» Product Name: Anti-phospho-Chk2 (Thr68), clone E126, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Rabbit View All»
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