货号：MAB4072

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Bromodeoxyuridine Antibody, clone 131-14871 | MAB4072 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes BrdU (Bromodeoxyuridine). View All» Clone: 131-14871 View All» Isotype: IgG1 View All» Background Information: The thymidine analog 5-bromo-2′-deoxyuridine (BrdU) is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. BrdU is readily incorporated into newly synthesized DNA, labeling cells that have progressed through the S-phase of the cell cycle and thereby serving as a marker for proliferating cells. View All» Species Reactivity: All View All» Application Notes: Immunohistochemistry on deparaffinized sections of kidney, duodenum, and liver tissue: 1:1000-1:1250. Flow cytometry on 100μL of 106 cells: 1:5000.Optimal dilutions must be determined by the end user. IMMUNOHISTOCHEMICAL PROCEDURE FOR PARAFFIN EMBEDDED TISSUE SECTIONS STAINING PROCEDURE: 1. Deparaffinize sections in xylene 3 x 5 minutes. Resin embedded or GMA (Glycol methacrylate, 2-hydroxyethyl methacrylate) treated sections it is unnecessary to deparaffinize as there is no paraffin. 2. Place slides in graded alcohols (100, 95, 90%) to water at 2 minute intervals, and air dry. (Omit for GMA sections) 3. Circle sections with PAP pen or diamond pen to identify and dry thoroughly. 4. For GMA or resin sections, place slides in 0.5% Tween/PBS (pH 7.6) for 29 minutes. 5. Incubate in prewarmed (40°C) 1N HCl for 1 hour. 6. Wash 3 x 3 minutes with PBS (pH 7.6). Slides may be held at this point and the procedure continued the following day. 7. Incubate in 1X Trypsin solution (0.02-0.05% w/v, prewarmed to 40°C) for 20 minutes at room temperature for NBF (normal buffered formalin) fixed tissue or 10 minutes for Carnoy's fixed tissue. 8. Wash 3 x 5 minutes with PBS (pH 7.6). 9. Incubate sections in 1% H2O2 (10 mL 30% H2O2 in 290 mL methanol) for 20 minutes, or GMA sections in 3% H2O2 (10 mL 30% H2O2 in 90 mL water) for 3 minutes. 10. Wash 2 x 2 minutes with PBS (pH 7.6). 11. Using the Vector ABC kit, add 3 drops of normal horse serum to 10 mL of PBS. Use this solution to block the slides for 20 minutes at room temperature. 12. Blot the slides of excess normal horse serum and incubate with MAB4072 diluted in PBS/BSA/Tween 20 for 1 hour at room temperature. 13. Wash 3 x 3 minutes with PBS (pH 7.6). 14. Add 3 drops of normal horse serum to 10 mL of PBS and then add 1 drop of biotinylated antibody stock. Incubate the slides with this diluted second antibody for 30 minutes at room temperature. 15. Prepare the Vectastain ABC Reagent by adding 2 drops of reagent A to 5 mL of PBS. Then add 2 drops of Reagent B to the same mixing bottle. Allow to sit for about 30 minutes prior to use. 16. Wash 3 x 3 minutes with PBS (pH 7.6). 17. Incubate the slides with ABC Reagent for 30 minutes at room temperature. 18. Wash 3 x 3 minutes with PBS (pH 7.6). 19. Prepare the substrate by mixing an equal volume of 0.02% H2O2 (made in distilled water from a 30% stock solution) and 0.1% DAB made in 0.1 M Tris buffer, pH 7.2. The H2O2 should be freshly prepared from concentrated stock. Because many peroxide substrates are unstable in the presence of H2O2 or when exposed to light, the substrate should be prepared just prior to use. Since DAB is a suspected carcinogen, care should be taken in handling and disposing of all peroxidase substrates. 20. Stain the slides with DAB substrate for 2-5 minutes. 21. Wash 2 x 2 minutes with distilled water. 22. Counterstain with hemotoxylin, dehydrate through xylene, and mount the coverslip. View All» Control: Brdu treated cells View All» Purification Method: Protein A purfied View All» Presentation: Liquid in 10 mM Phosphate buffer, pH 7.4 containing 150 mM NaCl and 0.1% sodium azide. View All» Storage Conditions: Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» Alternate Names: BrdU View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Flow Cytometry

• Immunohistochemistry

• Immunohistochemistry (Paraffin)
  View All» Brand Family: Chemicon View All» Product Name: Anti-Bromodeoxyuridine Antibody, clone 131-14871 | MAB4072 View All» Concentration: 1.1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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  © Merck Millipore代理MAB4072 Anti-BrdU Antibody, clone 131-14871;抗-BrdU抗体，克隆131-14871store at -20℃，产品报价联系微信jinshanbio