货号：MAB4092

品牌：Merck Millipore

规格：100Ug

目录价：￥4746.00

市场价格：￥4034.10

会员价格：￥3796.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 | MAB4092 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}. View All» Immunogen: The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). View All» Clone: KPL-1 View All» Isotype: IgG1κ View All» Background Information: Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}. View All» Species Reactivity: Human View All» Species Reactivity Note: Known not to react with Rat, Bovine, Pig, and Equine PSGL-1 {Snapp, KR et al 1998; Baisse, B et al 2007}. View All» Application Notes: Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000FACS {Snapp, KR et al 1998}. Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).Functional blocking of P-selectin {Snapp KR et al 1998}.Immunohistochemistry: Fresh frozen, acetone fixed sectionsImmunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200Optimal working dilutions must be determined by the end user. View All» Presentation: Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots up to 12 months. View All» UniProt Number: Q14242 View All» Entrez Gene Number: NM_003006.3 View All» Gene Symbol:

• SELPLG

• CLA

• PSGL-1

• CD162

• PSGL1
  View All» Alternate Names: PSGL-1; CD162 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Flow Cytometry

  • Immunohistochemistry

  • Immunohistochemistry (Paraffin)

  • Immunoprecipitation

  • Western Blotting
    View All» Entrez Gene Summary: SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters. View All» UniProt Summary: FUNCTION: SwissProt: Q14242 # Binds to P-, E- and L-selectins. The calcium-dependent high affinity interaction with P-selectin mediates the tethering and rolling of neutrophils and T-lymphocytes on endothelial cells.SIZE: 412 amino acids; 43201 Da SUBUNIT: Homodimer; disulfide-linked.SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes.PTM: Displays complex, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Also N- glycosylated. & Sulfated in the N-terminal region; sulfation is necessary for P-selectin binding. View All» Brand Family: Chemicon View All» Product Name: Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 | MAB4092 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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