货号：04-1524

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cdt1 Antibody, clone PKS136, 1-104 | 04-1524 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Recognizes Cdt1 View All» Molecular Weight: ~60 kDa View All» Epitope: 0 View All» Immunogen: His-tagged recombinant human protein corresponding to human Cdt1. View All» Clone: PKS136, 1-104 View All» Isotype: IgG2bκ View All» Background Information: Cdt1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. Cdt1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, Cdt1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. Cdt1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006). View All» Species Reactivity: Human View All» Species Reactivity Note: Reacts with human. View All» Application Notes: Immunoprecipitation: A previous lot of this antibody was used in IP. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: 0.5 µg/mL of this antibody detected Cdt1 on 10 µg of HeLa cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q9H211 View All» Entrez Gene Number: NP_112190.2 View All» Gene Symbol:

• CDT1

• DUP

• RIS2
  View All» Alternate Names:
  • DNA replication factor

  • Double parked homolog

  • Double parked, Drosophila, homolog of

  • chromatin licensing and DNA replication factor 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation
      View All» UniProt Summary: FUNCTION: Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene. SUBUNIT STRUCTURE: Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. SUBCELLULAR LOCATION: Nucleus. DEVELOPMENTAL STAGE: Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases. PTM : Ubiquitinated, leading to its subsequent degradation. The interaction with GMNN protects it against ubiquitination. Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.SEQUENCE SIMILARITIES: Belongs to the Cdt1 family.SEQUENCE CAUTION: The sequence AF070552 differs from that shown. Reason: Frameshift at positions 278 and 312. View All» Product Name: Anti-Cdt1, clone PKS136, 1-104 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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