货号：MAB4130

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Thymidylate Synthase Antibody, clone TS106 | MAB4130 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Antibody MAB4130 recognizes a 36 kDa protein identified as Thymidylate Synthase [TS], which is a target for the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. Expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas. View All» Molecular Weight: 36 kDa View All» Immunogen: Recombinant Thymidylate Synthase. View All» Clone: TS106 View All» Isotype: IgG1 View All» Background Information: TS (EC:2.1.1.45), a cytosolic enzyme, is a dimmer of two identical monomers of about 36kDa. The enzyme provides the sole intracellular de novo source of thymidylate and plays a crucial role in DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dump) and its conversion to deoxythymidine monophosphate (dTMP). Therefore, TS is primarily active in proliferating and metabolic active cells. TS is a central target of the widely used antineoplastic agent 5-Fluorouracil (5-FU) and thus also of the Xeloda, which is enzymatically activated to 5-FU. TS is inactivated by a covalent complex formation with 5-FdUMP and methylenetetrahydrofolate. Literature indicates that expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas with low TS expression predicting better response to 5-FU and survival. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human and mouse.Expected to react with rat based on sequence homology. View All» Application Notes: ELISA: A previous lot of this antibody was used in ELISA.Flow Cytometry: A previous lot of this antibody was used in FC.Immunofluorescence: A previous lot of this antibody was used in IF.Immunoprecipitation: A 10 μL/mg concentration of a previous lot was used on protein lysate.Immunohistochemistry (Frozen and Paraffin sections): 1:50 - 1:100 (with incubation for 30-60 minutes at room temperature). TS106 can benefit from antigen retrieval with either EDTA pH 8.0 or 10mM citrate buffer pH 6.0 . Western Blotting: 1:200 - 1:400. Recognizes a 36KDa band.Optimal working dilutions must be determined by end user. View All» Control: Positive Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10 View All» Quality Assurance: Evaluated by Western Blot on MCF7 lysates.Western Blot Analysis: 1:500 dilution of this antibody detected THYMIDYLATE SYNTHASE on 10 μg of MCF7 lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1 in 0.02M PBS with 0.25M NaCl, pH=7.6, 0.1% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt. View All» UniProt Number: P04818 View All» Entrez Gene Number: NM_001071.1 View All» Gene Symbol:
  • TYMS

  • TS

  • Tsase

  • TSase

  • TMS

  • HsT422

  • MGC88736

  • EC 2.1.1.45
    View All» Alternate Names: Thymidylate synthase View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Flow Cytometry

    • Immunofluorescence

    • Immunohistochemistry

    • Immunohistochemistry (Paraffin)

    • Immunoprecipitation

    • Western Blotting
      View All» Entrez Gene Summary: Thymidylate synthase (TYMS, or TS; EC 2.1.1.45) uses the 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor to maintain the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.[supplied by OMIM] View All» UniProt Summary: SIZE: 313 amino acids; 35716 Da SUBUNIT: Homodimer.SIMILARITY: SwissProt: P04818 ## Belongs to the thymidylate synthase family. View All» Brand Family: Chemicon View All» Product Name: Anti-Thymidylate Synthase Antibody, clone TS106 | MAB4130 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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