货号：04-199

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-ATF-2 (Thr71) Antibody, clone E268, Rabbit | 04-199 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes phosphorylated residues surrounding Thr71 of human ATF-2. View All» Molecular Weight: 70 kDa View All» Epitope: Thr71 View All» Immunogen: Synthetic peptide directed against the region encompassing and including phosphorylated ATF2 (Thr71). View All» Modifications: Phosphorylation View All» Clone: E268 View All» Isotype: IgG View All» Background Information: The ATF/CREB family of DNA binding proteins consists of transcription factors whose activity is regulated by specific signaling pathways, including the stress/cytokine/radiation inducible pathways that function through SAPK1/JNK activation. Two threonine residues at positions 69 and 71 in the N-terminal region of ATF-2 are essential in mediating adenovirus E1A-inducible transcriptional activation. These residues are efficiently phosphorylated in vivo and are excellent substrates for SAPK1/JNK in vitro. View All» Species Reactivity: Human View All» Species Reactivity Note: Proven to react with Human. Not expected to react with rat and mouse View All» Non-Reactive Species:

• Mouse

• Rat
  View All» Application Notes: Immunofluorescent Immunocytochemistry:Immunoprecipitation: A previous lot of this antibody was used in IP. View All» Control: Untreated and Calyculin/Okadaic acid treated HeLa Cell Lysate. View All» Quality Assurance: Routinely evaluated by Western Blot on untreated and Anisomycin treated HeLa cell lysate.Western Blot Analysis: A 1:2500-1:5000 dilution of this lot was used to detect ATF-2 in untreated and Anisomycin treated HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. View All» UniProt Number: P15336 View All» Entrez Gene Number: NM_001880.2 View All» Gene Symbol:
  • CRE-BP1

  • CREB2

  • CREBP1

  • HB16

  • MGC111558

  • OTTHUMP00000163262

  • TREB7
    View All» Alternate Names:
    • activating transcription factor 2

    • activating transcription factor 2 splice variant ATF2-var2

    • cAMP response element-binding protein CRE-BP1

    • cAMP responsive element binding protein 2

    • cAMP responsive element binding protein 2, formerly
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting

      • Immunocytochemistry
        View All» Entrez Gene Summary: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq]. View All» UniProt Summary: FUNCTION:Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.SUBUNIT: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Ref.5SUBCELLULAR LOCATION: Nucleus. SPECIFICITY: Abundant expression seen in the brain.PTM: Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. Ref.6 Ref.7 Ref.8 Ref.9SIMILARITY: Belongs to the bZIP family. ATF subfamily.Contains 1 bZIP domain.Contains 1 C2H2-type zinc finger.CAUTION: It is uncertain whether Met-1 or Met-19 is the initiator. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-ATF-2 (Thr71), clone E268, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Ascites View All» Host: Rabbit View All»
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