货号：04-205

品牌：Merck Millipore

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目录价：￥4376.00

市场价格：￥3719.60

会员价格：￥3500.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CENP-A Antibody, clone EP800Y | 04-205 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes the C-terminal region of human CENP-A. View All» Molecular Weight: 18 kDa View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to the C-terminal region of CENP-A, conjugated to KLH. View All» Clone: EP800Y View All» Isotype: IgG View All» Background Information: Centromere protein A (CENP-A) is a 17 kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of α-satellite DNA into primary chromatin structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker. View All» Species Reactivity:

• Human

• Rat
  View All» Species Reactivity Note: Proven to react with human and rat CENP-A. Does not react with mouse. View All» Non-Reactive Species: Mouse View All» Application Notes: Flow Cytometry: 1:80 dilution of a previous lot was used for flow cytometry.Immunocytochemistry: 1:250 – 500 dilution of a previous lot was used for Immunocytochemistry.Immunohistochemistry: 1:250 – 500 dilution of a previous lot was used for Immunohistochemistry.Immunocytochemistry:A previous lot was used to detect CENP-A in HeLa cells (1:250 dilution). View All» Control: HeLa Whole Cell lysate. View All» Quality Assurance: Routinely evaluated by Western Blot on HeLa Whole Cell lysate.Western Blot Analysis:A 1:5,000 dilution of this lot was used to detect CENP-A HeLa Whole Cell lysate. View All» Purification Method: Unpurified View All» Presentation: Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P49450 View All» Entrez Gene Number: NM_001042426 View All» Gene Symbol:
  • CENP-A

  • OTTHUMP00000122589
    View All» Alternate Names:
    • Centromere autoantigen A

    • centromere protein A

    • centromere protein A (17kD)

    • centromere protein A, 17kDa
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Flow Cytometry

      • Immunocytochemistry

      • Immunohistochemistry

      • Immunohistochemistry (Paraffin)

      • Western Blotting
        View All» Entrez Gene Summary: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq] View All» UniProt Summary: FUNCTION: Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.SUBUNIT: Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with herpes virus HSV-1 ICP0 protein. Ref.5 Ref.7 Ref.12SUBCELLULAR LOCATION: Nucleus. Centromere. Kinetochore. Note= Localizes exclusively in the kinetochore domain of centromeres. Ref.10 Ref.11DOMAIN: The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres. Ref.12PTM: Ubiquitinated Probable. Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.DISEASE:Antibodies against CENPA are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins. Ref.6SIMILARITY: Belongs to the histone H3 family. View All» Brand Family: Upstate View All» Product Name: Anti-CENP-A, clone EP800Y View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Unpurified View All» Host: Rabbit View All»
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