货号：MAB429

品牌：Merck Millipore

规格：

目录价：￥3908.00

市场价格：￥3321.80

会员价格：￥3126.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Progesterone Receptor Antibody, clone 1A6 | MAB429 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Human progesterone receptor protein. STAINING PATTERN: Nuclear View All» Immunogen: Synthetic peptide corresponding to a site of predicted high antigenicity on the human progesterone receptor. View All» Clone: 1A6 View All» Isotype: IgG1 View All» Species Reactivity: Human View All» Application Notes: Immunohistochemistry Frozen sections: 1:40, (fixed for 10 minutes at room temperature with Zamboni's) 60 minutes incubation at 25°C. Indirect immunoperoxidase technique. INDIRECT IMMUNOPEROXIDASE TECHNIQUE FOR MAB429 1. Cut 7 micron sections of frozen tissue and fix immediately in Zamboni's fixative for 10 min. 2. Wash 3 x 10 min. in TBS. 3. Cover with NGS for 10 min. 4. Remove excess serum, cover with MAB429 (Diluted 1:40 with NGS) and incubate 1 hour at room temp. 5. Wash in TBS for 2 x 5 min. 6. Cover with secondary antibody and incubate for 30 min. at room temperature. 7. Wash in TBS for 2 x 5 min. 8. Develop with DAB. 9. It is suggested that a counterstain is not used. 10. Dehydrate, clear and mount. *Note: NGS = Normal Goat Serum diluted 1 in 5 with TBS. ZAMBONI'S FIXATIVE 1. Mix 20 G paraformaldehyde with 150 mL double-filtered, saturated aqueous picric acid. 2. Heat to 60° C in fume cupboard. 3. Add 2.52% sodium hydroxide in water, drop by drop until solution is clear. 4. Filter solution and allow to cool. 5. Make up to 1000 mL with phosphate buffer. (3.31g NaH2 PO4.H2O, 33.77g Na2HPO4.7H2O 1000 mL) Reference: 1) Nature (1967) 216:174-175 Paraffin embedded sections: 1:40 with high temperature antigen unmasking technique. 60 minutes incubation at 25°C. Standard ABC technique. Western blotting: 1:25-1:50 Optimal working dilutions must be determined by the end user. View All» Control: POSITIVE CONTROL: Lobular and tubular breast carcinomas tend to be positive. View All» Presentation: Culture supernatant, with 15 mM sodium azide, liquid. View All» Storage Conditions: Store at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P06401 View All» Entrez Gene Number: NM_000926.4 View All» Gene Symbol:

• PGR

• PR

• NR3C3
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Immunohistochemistry

  • Immunohistochemistry (Paraffin)
    View All» Entrez Gene Summary: This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform B has not been clearly determined. View All» UniProt Summary: FUNCTION: SwissProt: P06401 # The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.SIZE: 933 amino acids; 98981 Da SUBUNIT: Interacts with CUEDC2, SMARD1 and with UNC45A.SUBCELLULAR LOCATION: Nucleus.DOMAIN: SwissProt: P06401 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Progesterone Receptor Antibody, clone 1A6 | MAB429 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 500 µL View All» Format: Culture Supernatant View All» Host: Mouse View All»
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