货号：04-218

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CREB Antibody, clone E306, rabbit | 04-218 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes CREB. View All» Molecular Weight: ~43 kDa View All» Epitope: Internal View All» Immunogen: KLH conjugated to CREB linear peptide. View All» Clone: E306 View All» Isotype: IgG View All» Background Information: CREB, cyclic AMP (cAMP) response element-binding protein, is a ubiquitously expressed basic leucine zipper transcription factor that functions in glucose homeostasis and stimulates the expression of numerous genes in response to growth factors, hormones, neurotransmitters, ion fluxes, stress signals, and has been implicated in memory because if its apparent role in synaptic plasticity. CREB functions either by itself or with related family members, which include CREB, CREM, and ATF-1, by forming homo- and heterodimers through their basic leucine zipper (bZIP) domains. The CREB signaling pathway is activated by extracellular ligands that bind to cell surface receptors and relay their message through various intracellular second messengers resulting ultimately in the activation of nuclear CREB through phosphorylation of the transactivation domain at serine 1332,4. The classical model of CREB activation is through PKA (cAMP-dependent protein kinase). In the non-active state, PKA resides in the cytoplasm as an inactive heterotetramer of paired regulatory and catalytic subunits. Stimulation of cAMP causes the releases of the catalytic subunits of PKA that passively diffuse into the nucleus and initiates gene expression by phosphorylating CREB at its transactivation domain on serine 1331,2,3,4. CREB mediates the activation of cAMP-responsive genes by binding as a dimer to a conserved cAMP-responsive element (CRE), 5’-TGACGTCA-3’, located within the promoter region of target genes. Target gene activation is terminated by the serine/threonine phosphatases PP-1-mediated dephospho-rylation of CREB. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Proven to react with human, rat and mouse. View All» Application Notes: Flow Cytometry: 1:40 dilution from a previous lot was used on flow cytometry.Immunohistochemistry: 1:250-500 dilution from a previous lot was used on immunohistochemistry.Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.Immunocytochemistry: 1:250-500 dilution from a previous lot was used on immunocytochemistry. View All» Control: A431 whole cell lysate. View All» Quality Assurance: Routinely evaluated by Western Blot on A431 whole cell lysate.Western Blot Analysis:A 1:1000 dilution of this lot was used to detect CREB in A431 whole cell lysate. View All» Presentation: 100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P16220 View All» Entrez Gene Number: NM_004379 View All» Gene Symbol:
  • MGC9284

  • CREB
    View All» Alternate Names:
    • cAMP responsive element binding protein

    • active transcription factor CREB

    • transactivator protein
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Flow Cytometry

      • Immunocytochemistry

      • Immunofluorescence

      • Immunohistochemistry (Paraffin)

      • Immunoprecipitation

      • Western Blotting
        View All» UniProt Summary: Function: This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP By similarity.Subcellular location: Nucleus. Post-translational modification: Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP domain.Contains 1 KID (kinase-inducible) domain. View All» Brand Family: Upstate View All» Product Name: Anti-CREB, clone E306, rabbit monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-131607.htm
        © Merck Millipore代理04-218 Anti-CREB, clone E306 (rabbit monoclonal)，产品报价联系微信jinshanbio