货号：04-222

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cyclin E1 Antibody,clone EP435E rabbit | 04-222 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes Cyclin E1. View All» Molecular Weight: 50 kDa View All» Epitope: Internal View All» Immunogen: Synthetic peptide identical to an internal region of human Cyclin E1. View All» Clone: EP435E View All» Isotype: IgG View All» Background Information: Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature. View All» Species Reactivity: Human View All» Species Reactivity Note: Proven to react with human. Not expected to react with rat or mouse. View All» Non-Reactive Species:

• Mouse

• Rat
  View All» Application Notes: Immunocytochemistry: 1:100-250 dilution of a previous lot was used on immunocytochemistry.Immunoprecipitation: 1:80 dilution of a previous lot was used on immunoprecipitation.Flow Cytometry: 1:20 dilution of a previous lot was used on flow cytometry. View All» Control: HeLa whole cell lysate. View All» Quality Assurance: Routinely evaluated by Western Blot on HeLa whole cell lysate.Western Blot Analysis:A 1:1000-1:2000 dilution of this lot was used to detect Cyclin E1 on HeLa whole cell lysate. View All» Purification Method: Unpurified View All» Presentation: Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P24864 View All» Entrez Gene Number: NM_001238.1 View All» Gene Symbol: CCNE View All» Alternate Names:
  • cyclin E1

  • cyclin Es

  • cyclin Et
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Flow Cytometry

    • Immunocytochemistry

    • Immunoprecipitation

    • Western Blotting
      View All» Entrez Gene Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq] View All» UniProt Summary: Function: Essential for the control of the cell cycle at the G1/S (start) transition.Subunit structure: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1 By similarity. Part of a complex consisting of UHRF2, CDK2 and CCNE1.Subcellular location: Nucleus. Tissue specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Ref.10Post-translational modification: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.9 Ref.11 Ref.13 Ref.14Sequence similarities: Belongs to the cyclin family. Cyclin E subfamily View All» Brand Family: Upstate View All» Product Name: Anti-Cyclin E1,clone EP435E rabbit monoclonal Antibody View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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