货号:MAB4309
品牌:Merck Millipore
规格:EA
目录价:¥3779.00
市场价格:¥3212.15
会员价格:¥3023.20
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Description:
Anti-VIN-2PB-22 Antibody, clone VIN-2PB-22 | MAB4309
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Trade Name:
Chemicon (Millipore)
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Specificity:
This monoclonal antibody recognizes a ganglioseries glycolipid, gangliosides GD2. Experimental evidence suggests that the expression of this antigen increases following human Embryonal Carcinoma and Embryonic Stem cell differentiation. It is thought that this antibody recognizes cells of the neuroectoderm lineage.
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Immunogen:
Human Embryonal carcinoma cells
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Clone:
VIN-2PB-22
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Isotype:
IgM
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Background Information:
GD2 is a disialoganglioside expressed on tumors of neuroectodermal origin, including human neuroblastoma and melanoma, with highly restricted expression on normal tissues, principally to the cerebellum and peripheral nerves in humans. The relatively tumor specific expression of GD2 makes it a suitable target for immunotherapy with monoclonal antibodies or with artificial T cell receptors.
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Species Reactivity:
Mammals
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Application Notes:
Immunohistochemistry & Immunocytochemistry: Suggested fixatives: Paraformaldehyde is recommended, NOT methanol or acetone (the glycolipid antigen is extracted by polar lipid solvents). Recommended antibody dilution: 10-20 μg/ml Dilution Buffer: PBS + 4% FCS Expected staining pattern: cell surface staining FACS Analysis: Suggested dilution/number of cells: 10-20 μg/ml for 1 x 105 cellsFixation/Permeabilization used: Live cells or fixed in 4% Paraformaldehyde Recommended controls: Undifferentiated human ES or EC cells stain weakly. Immunoblotting: Not tested EIA: Not tested Optimal working dilutions must be determined by end user.
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Control:
FACS Analysis: Undifferentiated human embryonic stem or embryonic carcinoma cells stain weakly
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Presentation:
Purified immunoglobulin. Liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide
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Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
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Gene Symbol:
VIN-2PB-22
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Flow Cytometry
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Immunocytochemistry
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Immunohistochemistry
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Brand Family:
Chemicon
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Product Name:
Anti-VIN-2PB-22 Antibody, clone VIN-2PB-22 | MAB4309
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Concentration:
1 mg/mL
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Antibody Type:
Monoclonal Antibody
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Qty/Pk:
100 µg
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Format:
Purified
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Host:
Mouse
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