货号：04-226

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Elk1 Antibody, (C-term), clone E277, rabbit | 04-226 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes Elk-1 at the C-Terminus. The antibody does not cross-react with other ETS family members. View All» Molecular Weight: 47-60 kDa Molecular weight varies depending on modifications. View All» Epitope: C-terminus View All» Immunogen: KLH conjugated linear peptide of Elk-1. View All» Clone: E277 View All» Isotype: IgG View All» Background Information: ELK1 is a transcription factor and a member of the Ets family of transcription factors. Elk1 is a downstream transcription factor of the Ras-Raf-MEK-Erk (canonical MAPK) pathway. View All» Species Reactivity: Human View All» Species Reactivity Note: Proven to react with human. Not expected to react with mouse and rat. View All» Application Notes: Immunohistochemistry: 1:50 dilution from a previous lot was used on immunohistochemistry.Immunocytochemistry: 1:100 dilution from a previous lot was used on immunocytochemistry.Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation. View All» Control: A431 whole cell lysate. View All» Quality Assurance: Routinely evaluated by Western Blot on A431 whole cell lysate.Western Blot Analysis:A 1:500 dilution of this lot was used to detect Elk-1 on A431 whole cell lysate. View All» Presentation: Unpurified rabbit monoclonal IgG in buffer contaiing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P19419 View All» Entrez Gene Number: NM_005229 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunocytochemistry

• Immunohistochemistry

• Immunohistochemistry (Paraffin)

• Immunoprecipitation

• Western Blotting
  View All» Entrez Gene Summary: This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] View All» UniProt Summary: Function: Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.Subunit structure: Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Ref.12Subcellular location: Nucleus. Tissue specificity: Lung and testis.Post-translational modification: Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Ref.7Sequence similarities: Belongs to the ETS family.Contains 1 ETS DNA-binding domain. View All» Brand Family: Upstate View All» Product Name: Anti-Elk1, (C-term), clone E277, rabbit monoclonal Antibody View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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