货号：04-236

品牌：Merck Millipore

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目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Nibrin/NBS1 Antibody, clone Y112 | 04-236 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes Nibrin/NBS1. View All» Molecular Weight: 95 kDa View All» Epitope: C-terminus View All» Immunogen: KLH conjugated to Nibrin/NBS1 linear peptide. View All» Clone: Y112 View All» Isotype: IgG View All» Background Information: Nibrin (NBS1, NBN) is known to be a key regulator of DNA Damage repair by forming a complex with Rad50 and MRE11. This complex, more commonly referred to as MRN (Mre11/Rad50/Nibrin) is important in double-stranded break repair, DNA recombination, cell cycle checkpoint control, and meiosis. This complex quickly recognizes double stranded break (DSB) sites and quickly relocates to these sites. Nibrin senses DNA damage by recruiting ATM, ATR, and DNA-PK to the damage sites and activation their functions. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Proven to react with human and mouse. Not expected to react with rat. View All» Application Notes: Immunohistochemistry: A 1:50 dilution of a previous lot was used in IH.Immunoprecipitation: A 1:60 dilution of a previous lot was used in IP.Immunocytochemistry: A 1:50 dilution of a previous lot was used in IC. View All» Control: HeLa whole cell lysate. View All» Quality Assurance: Routinely evaluated by Western Blot on HeLa whole cell lysate.Western Blot Analysis: A 1:250 dilution of this lot was used to detect Nibrin/NBS1 in HeLa whole cell lysate. View All» Purification Method: Unpurified View All» Presentation: Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O60934 View All» Entrez Gene Number: NM_002485.4 View All» Gene Symbol:
  • AT-V1

  • AT-V2

  • ATV

  • FLJ10155

  • MGC87362

  • NBS

  • NBS1

  • P95

  • nibrin
    View All» Alternate Names:
    • Cell cycle regulatory protein p95

    • Nijmegen breakage syndrome 1 (nibrin)

    • Nijmegen breakage syndrome protein 1

    • p95 protein of the MRE11/RAD50 complex
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunohistochemistry

      • Immunohistochemistry (Paraffin)

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq] View All» UniProt Summary: Function: Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Ref.9 Ref.14 Ref.19Subunit structure: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A By similarity. Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with HJURP, KPNA2 and TERF2.Subcellular location: NucleusBy similarity. TelomereBy similarity. Note= Localizes to discrete nuclear foci after treatment with genotoxic agents By similarity.Tissue specificity: Ubiquitous. Expressed at high levels in testis.Domain: The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage. Ref.18The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex. Ref.18The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response. Post-translational modification: Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance. Involvement in disease: Defects in NBN are the cause of Nijmegen breakage syndrome (NBS) [MIM:251260]. NBS is an autosomal recessive syndrome characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies.Defects in NBN are a cause of genetic susceptibility to breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Defects in NBN may be associated with aplastic anemia [MIM:609135]. Aplastic anemia is a disease of bone-marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. Most of the cases of aplastic anemia are idiopathic, some are familial and some are due to a viral infection or to exposure to chemicals and radiation. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).Miscellaneous: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.Sequence similarities: Contains 1 BRCT domain.Contains 1 FHA domain.Sequence caution: The sequence AAI08651.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence starting in position 550. View All» Brand Family: Upstate View All» Product Name: Anti-Nibrin/NBS1, clone Y112 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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