货号：MAB4334Z

品牌：Merck Millipore

规格：100Ug

目录价：￥4834.00

市场价格：￥4108.90

会员价格：￥3867.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MDR1 Antibody, clone UIC2, azide free | MAB4334Z View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243) View All» Epitope: conformational extracellular epitope, azide free View All» Immunogen: Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine. View All» Clone: UIC2 View All» Isotype: IgG2aκ View All» Background Information: UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE's UIC2 Shift assay - see product ECM905). View All» Species Reactivity:

• Human

• Primate
  View All» Non-Reactive Species:
  • Mouse

  • Rat
    View All» Application Notes: Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 ug per 1 μl of cell lysate Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors IHC on frozen and paraffin sections: 1 - 10 μg/mL View All» Presentation: Purified immunoglobulin from Protein A Sepharose™ chromatography. Sterile filtered in 0.01 M PBS, no preservative. View All» Storage Conditions: Maintain at 2-8°C for up 12 months. View All» UniProt Number: P08183 View All» Entrez Gene Number: NM_000927.3 View All» Gene Symbol:
    • ABCB1

    • PGY1

    • MGC163296

    • CD243

    • GP170

    • P-gp

    • MDR1

    • CLCS

    • ABC20

    • EC 3.6.3.44
      View All» Alternate Names:
      • P-glycoprotein

      • CD243

      • p170

      • Pgp

      • MAB4334
        View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
        • Flow Cytometry

        • Immunofluorescence

        • Immunoprecipitation

        • Immunohistochemistry

        • Affects Function
          View All» Entrez Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. View All» UniProt Summary: FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.SIZE: 1280 amino acids; 141463 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains. View All» Brand Family: Chemicon View All» Product Name: Anti-MDR1 Antibody, clone UIC2, azide free | MAB4334Z View All» Concentration: 1mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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