货号：MAB4349

品牌：Merck Millipore

规格：100Ug

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells. View All» Molecular Weight: 212/80/55 kDa. View All» Epitope: Liver/Bone/Kidney Alkaline Phosphatase View All» Immunogen: 2102Ep human Embryonal Carcinoma Cells View All» Clone: clone TRA-2-49/6E View All» Isotype: IgG1 View All» Background Information: In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification. Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry. View All» Species Reactivity:

• Human

• Pig

• Rabbit

• Feline
  View All» Species Reactivity Note: Reactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger. No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP. View All» Non-Reactive Species:
  • Guinea Pig

  • Monkey

  • Mouse

  • Rat
    View All» Application Notes: Immunocytochemistry: A previous lot was used on IC.Immunofluorescence: A previous lot of this antibody was used in IF.FACS Analysis: A starting range of 5-20 µg/mL is suggested.Immunoprecipitation: A previous lot of this antibody was used in IP.Optimal working dilutions must be determined by end user. View All» Control: Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells) View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl pH 7.6 with 0.1% Sodium Azide View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» Gene Symbol: TRA-2-49 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Flow Cytometry

    • Immunofluorescence

    • Immunocytochemistry

    • Immunoprecipitation
      View All» Brand Family: Chemicon View All» Product Name: Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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