货号：MAB5236

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phosphacan View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Phosphacan (Receptor Tyrosine Phosphatase Beta). View All» Immunogen: Mixture of chicken chondroitin sulfate proteoglycans. View All» Isotype: IgG1 View All» Background Information: Phosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth. View All» Species Reactivity: Chicken View All» Application Notes: Western blot: Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples. ELISA: 1 μg/mL, excellent for core protein, good for monomer Immunocytochemistry: not tested Immunohistochemistry: does not work on fixed samples, unfixed has not been tested. Optimal working dilutions must be determined by the end user. View All» Presentation: Purified immunoglobulin. Liquid in PBS. Contains no preservative. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: Q62656 (rat) View All» Gene Symbol:

• PTPRZ1

• PTPRZ

• PTPZ
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • ELISA

  • Western Blotting
    View All» Brand Family: Chemicon View All» Product Name: Anti-Phosphacan View All» Concentration: 0.38 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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