货号：MAB5256X

品牌：Merck Millipore

规格：40Ug

目录价：￥6588.00

市场价格：￥5599.80

会员价格：￥5270.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor® 488 Conjugated | MAB5256X View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Neurofilament H. View All» Molecular Weight: ~ 200 kDa View All» Epitope: Unknown View All» Immunogen: Purified neurofilament polypeptides (Debus et al., 1983). View All» Clone: NE14 View All» Isotype: IgG1 View All» Background Information: Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure; however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. View All» Species Reactivity:

• Rat

• Human

• Pig
  View All» Species Reactivity Note: Human, Porcine and Rat. Reactivity with other species has not been tested. View All» Application Notes: Immunohistochemistry: 5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user. View All» Control: Rat Cortex Cells. View All» Quality Assurance: Immunocytochemical Analysis: 1:250 dilution of this lot detected Neurofilament-H in rat cortex cell culture. View All» Purification Method: Protein G purfied View All» Presentation: Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.1% sodium azide. View All» Storage Conditions: Stable for 6 months at 2-8ºC from date of receipt. View All» UniProt Number: P12036 View All» Entrez Gene Number: NP_066554.2 View All» Gene Symbol:
  • NEFH

  • NF-H

  • NFH
    View All» Alternate Names:
    • 200 kDa neurofilament protein

    • Neurofilament triplet H protein

    • neurofilament, heavy polypeptide

    • neurofilament, heavy polypeptide 200kDa
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Immunocytochemistry
        View All» Entrez Gene Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION:Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.POLYMORPHISM: The number of repeats is shown to vary between 29 and 30.DISEASE:Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. Ref.8SIMILARITY: Belongs to the intermediate filament family. View All» Product Name: Anti-Neurofilament H, clone NE14, Alexa Fluor® 488 Conjugated View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 40 μg View All» Format: Purified View All» Host: Mouse View All»
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