货号：MAB5290

品牌：Merck Millipore

规格：100Ug

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a | MAB5290 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Dopamine Receptor D1A, no cross reactivity to D1B (D5) receptor. Immunoreactivity was found to be specific for mouse and rat D1A receptor. Human, pig, dog, rabbit, sheep and bovine samples were non-reactive by western analysis. View All» Immunogen: Recombinant rat D1a Receptor, carboxy terminus. View All» Clone: SG2-D1a View All» Isotype: IgG2b View All» Species Reactivity:

• Mouse

• Rat

• Sheep
  View All» Non-Reactive Species:
  • Bovine

  • Canine

  • Human

  • Pig

  • Rabbit
    View All» Application Notes: Western blot: 1:250-1:500 on unboiled membrane fractions from rat caudate tissue. Reactivity is also seen in perfrontal and temporal cortex. An identified band between 45-66kDa is observed (Luedtke, 1999). Immunohistochemistry: 4% PFA fixed, vibratome sections, blocked with 3% NGS, 1% BSA with 0.2% triton X-100. Primary antibodies diluted onlyin PBS with excess blocking reagents. Optimal working dilutions must be determined by the end user. SUGGESTED WESTERN BLOT PROTOCOL Solutions 10X Transfer buffer 1 liter Tris 115.3 gm Glycine 24.2 gm [SDS 4 gm (may be omitted)] For a 1X working solution: 1 liter mix 700 mL water 200 mL MeOH 100 mL 10X TransferTBST stock 1 liter 1M Tris pH 7.5 10 mL 5 M NaC 30 mL 10% Tween-20 5 mL 1. Run SDS-PAGE gel as desired. 2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane. 3. Soak membrane in transfer buffer (see below) until ready to set up blot transfer (>15 min). 4. Assemble gel-transfer sandwich with the blot toward the positive electrode. 5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins. 6. Stain the transferred bands with Chemicon BLOT-FastStain™ (Catalog Number 2076). 7. Destain with deionized water. 8. Block with 5% non-fat milk (Marvel or Carnation) in TBS or PBS, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 9. Incubation with first antibody overnight with gentle agitation at 4°C. Optimal working dilutions and incubation time will need to be determined by the end user. 10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted. 11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature. 12. Wash at least 3 x 5 min. with TBST. 13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078). View All» Presentation: Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. View All» UniProt Number: P21728 View All» Entrez Gene Number: NM_000794.3 View All» Gene Symbol:
    • DRD1

    • DRD1A

    • DADR

    • D
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. View All» UniProt Summary: FUNCTION: SwissProt: P21728 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.SIZE: 446 amino acids; 49293 Da SUBUNIT: Interacts with DNAJC14 via its C-terminus (By similarity). Interacts with DRD1IP.SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14 (By similarity).TISSUE SPECIFICITY: Detected in caudate, nucleus accumbens and in the olfactory tubercle.SIMILARITY: SwissProt: P21728 ## Belongs to the G-protein coupled receptor 1 family. View All» Brand Family: Chemicon View All» Product Name: Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a | MAB5290 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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        © Merck Millipore代理MAB5290 Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a;抗-多巴胺D1A受体抗体，克隆SG2-D1astore at +2℃ to +8℃，产品报价联系微信jinshanbio