货号：MAB5292

品牌：Merck Millipore

规格：100Ug

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B | MAB5292 View All» Replaces: AB1791P View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Dopamine Receptor D1b (D5). View All» Immunogen: Recombinant rat D1b Receptor, carboxy terminus. View All» Clone: SG4-D1B View All» Isotype: IgG1 View All» Species Reactivity:

• Mouse

• Rat
  View All» Non-Reactive Species: Human View All» Application Notes: Western blot: 1:250-1:500 on mouse brain lysate (unboiled sample) Immunohistochemistry Optimal working dilutions must be determined by the end user. SUGGESTED WESTERN BLOT PROTOCOL Solutions 10X Transfer buffer 1 liter Tris 115.3 gm Glycine 24.2 gm [SDS 4 gm (may be omitted)] For a 1X working solution: 1 liter mix 700 mL water 200 mL MeOH100 mL 10X Transfer TBSTstock 1 liter1M Tris pH 7.5 10 mL 5 M NaCl 30 mL 10% Tween-20 5 Ml 1. Run SDS-PAGE gel as desired. 2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane. 3. Soak membrane in transfer buffer (see below) until ready to set up blot (>15 min) transfer. 4. Assemble gel-transfer sandwich with the blot toward the positive electrode. 5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins. 6. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076). 7. Destain with deionized water. 8. Block with 5% non-fat milk (Marvel or Carnation) in water, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc). 9. Incubation with first antibody overnight with rocking at 4°C in blocking solution. Optimal working dilutions and incubation time will need to be determined by the end user. 10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted. 11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature. 12. Wash at least 3 x 5 min. with TBST. 13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078). View All» Presentation: Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. View All» UniProt Number: P21918 View All» Entrez Gene Number: NM_000798.3 View All» Gene Symbol:
  • DRD5

  • DRD1B

  • DRD1L2

  • DBDR

  • D

  • MGC10601
    View All» Alternate Names: Dopamine D5 Receptor View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry

    • Western Blotting
      View All» Entrez Gene Summary: This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. View All» UniProt Summary: FUNCTION: SwissProt: P21918 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.SIZE: 477 amino acids; 52951 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Neuron-specific, localized primarily within limbic regions of the brain.DISEASE: SwissProt: P21918 # Defects in DRD5 may be a cause of schizophrenia, but no proof has yet been found. & Defects in DRD5 are a cause of blepharospasm [MIM:606798]. It is a primary focal dystonia affecting the orbicularis oculi muscles, usually beginning in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. In severe cases, this can lead to functional blindness.SIMILARITY: SwissProt: P21918 ## Belongs to the G-protein coupled receptor 1 family. View All» Brand Family: Chemicon View All» Product Name: Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B | MAB5292 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Target / Localization: Cytoplasmic View All» Format: Purified View All» Host: Mouse View All»
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