货号：MAB5324

品牌：Merck Millipore

规格：50UL

目录价：￥7219.00

市场价格：￥6136.15

会员价格：￥5775.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Polysialic Acid-NCAM Antibody, clone 2-2B | MAB5324 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with alpha 2-8 linked neuraminic acid (NeuAc-alpha 2-8) n with n >10. This polymer is usually termed polysialic acid (PSA). In vertebrates PSA is linked to neural cell adhesion molecule (NCAM, CD56), in bacteria it is associated with capsula of meningococcus strain group B. View All» Molecular Weight: 150-300 kDa View All» Immunogen: Viable Meningococcus group B (strain 355). View All» Clone: 2-2B View All» Isotype: IgM View All» Background Information: Polysialic acid (PSA) is a long, linear homopolymer that is attached to the neural cell adhesion molecule (NCAM). It has the remarkable ability to down-regulate a wide variety of contact-dependent cell interactions. This activity is believed to stem from the ability of the charged polymer to occupy a large hydrated volume and thereby cause a direct physical hindrance of cell–cell contact. The regulated expression of PSA has been shown to promote a reduction in cell interactions that can create permissive conditions for changes in the structure of a variety of tissues. The broadest expression of PSA occurs on precursor cells during early development and plays a role in promotion of the migration of these cells after cell division. In most cases, PSA expression is lost after the migration process is completed. However, the axons of neurons often retain PSA to bundle,sprout, and branch appropriately during axon path-finding. View All» Species Reactivity:

• Human

• Rat

• Mouse

• Vertebrates
  View All» Species Reactivity Note: The monoclonal works well on all species expressing PSA-NCAM including mouse. View All» Application Notes: Western Blotting: A 1:500-1:1,000 concentration of a previous lot was used in WB.Immunocyto/histochemistry: A 1:200-1:400 dilution of a previous lot of this antibody worked on live and fixed cells, tissue sections either frozen or fixed with any kind of fixative. (Rougon et al., 1986; Theodosis et al., 1991, 1999). It is not recommend that the antibody be used on paraffin embedded tissue since it gives variable results.Radioimmunoassay (RIA) : A previous lot of this antibody was used in RIA. (Figarella-Branger et al., 1996) Cell sorting and cell panning (Ben-Hur et al., 1998) Optimal working dilutions must be determined by end user. View All» Control: Positive control tissue: Young Rat Hippocampus, human hippocampus. View All» Quality Assurance: Routinely evaluated by Immunohistochemistry on hippocampus.Immunohistochemistry(paraffin) Analysis: PSA-NCAM (cat. # MAB5324) representative staining pattern/morphology on human hippocampus. Tissue pretreated with EDTA pH 8, antigen retrieval. Antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as plasma membrane staining in neurons.Optimal Staining With Epitope Retrieval: Alzheimer’s Hippocampus View All» Purification Method: Unpurified View All» Presentation: Mouse monoclonal IgM in buffer containing 50% glycerol, no preservatives. View All» Storage Conditions: Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P13591 View All» Entrez Gene Number: NM_000615.5 View All» Gene Symbol:
  • NCAM1

  • CD56

  • NCAM-140

  • NCAM

  • NCAM-120

  • MSK39
    View All» Alternate Names: PSA-NCAM View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry

    • Radioimmunoassay

    • Western Blotting
      View All» UniProt Summary: FUNCTION: SwissProt: P13592 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.| P13591 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.SIZE: 761 amino acids; 83770 Da SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.DOMAIN: SwissProt: P13592 SIMILARITY: SwissProt: P13592 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. | P13591 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. View All» Brand Family: Chemicon View All» Product Name: Anti-Polysialic Acid-NCAM Antibody, clone 2-2B | MAB5324 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 µL View All» Target / Localization: Plasma membrane View All» Format: Ascites View All» Host: Mouse View All»
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      © Merck Millipore代理MAB5324 Anti-Polysialic Acid-NCAM Antibody, clone 2-2B;抗-聚唾液酸-NCAM抗体，克隆2-2Bstore at -20℃，产品报价联系微信jinshanbio