货号：04-590

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Caldesmon Antibody, Rabbit, clone E89 | 04-590 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes Human, Mouse, Rat Caldesmon View All» Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon. View All» Clone: E89 View All» Background Information: Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, Mouse, Rat (positive control: NIH/3T3 cell lysate) View All» Application Notes: WB: 1:10,000-20,000 IHC: 1:250 (Paraffin-embedded human uterus) ICC: 1:250 (Hela cells) Flow Cytometry: 1:20 IP: 1:80 View All» Purification Method: Protein A purfied View All» Presentation: Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Store at -20 °C. Stable for 12 months from date of receipt. View All» UniProt Number: Q05682 View All» Entrez Gene Number:
  • NM_004342.5

  • NM_033138.2

  • NM_033139.2

  • NM_033140.2

  • NM_033157.2
    View All» Gene Symbol:
    • CALD1

    • CDM

    • CAD

    • L-CAD

    • MGC21352

    • Caldesmon

    • NAG22

    • H-CAD
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Flow Cytometry

      • Immunoprecipitation

      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. View All» UniProt Summary: FUNCTION: SwissProt: Q05682 # Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.SIZE: 793 amino acids; 93250 Da SUBCELLULAR LOCATION: Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) (By similarity).TISSUE SPECIFICITY: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.DOMAIN: SwissProt: Q05682 The N-terminal part seems to be a myosin/calmodulin- binding domain, and the C-terminal a tropomyosin/actin/calmodulin- binding domain. These two domains are separated by a central helical region in the smooth-muscle form.PTM: In non-muscle cells, phosphorylation by CDC2 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization (By similarity).SIMILARITY: Belongs to the caldesmon family. View All» Brand Family: Upstate View All» Product Name: Anti-Caldesmon Antibody, Rabbit, clone E89 | 04-590 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Rabbit View All»
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