货号：04-735

品牌：Merck Millipore

规格：100Ul

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314 | 04-735 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes monomethyl-histone H4 (Lys20), Mr ~10kDa. View All» Molecular Weight: 10kDa View All» Immunogen: KLH-conjugated, synthetic peptide containing the sequence …HR[mmK]V…, in which mmK corresponds to monomethyl-lysine at residue 20 of human histone H4. View All» Modifications: Methylation View All» Clone: NL314 View All» Isotype: IgG View All» Background Information: Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H4 on lys20 (H4-K20me3) selectively marks constitutive pericentromeric hetero-chromatin and imprinting control regions, while mono- and dimethylation are broadly distributed but principally enriched in euchromatin regions. Although the biological function of H4K20me remains poorly understood, studies suggest that H4-K20me2 is involved in the guidance of DNA repair proteins to DNA strand breaks, whereas H4K20me1 is associated with chromatin condensation processes. PR-Set7 (also known as SET8) specifically catalyzes monomethylation of H4K20me1. View All» Species Reactivity: Human View All» Species Reactivity Note: Human. The immunizing sequence is conserved from Tetrahymena to human, so broad species cross-reactivity is expected. View All» Application Notes: Immunoblot Analysis: A 1:4000 dilution of a previous lot detected methylated histone H3 in acid extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.Multiplexing: Beadlyte® Histone-Peptide Specificity Assay: 1:2,000-1:30,000 dilutions of a previous lot were incubated with histone H4 peptides containing various modifications conjugated to Luminex® microspheres. Specificity to monomethyl-histone H4 (Lys20) was demonstrated (Figure C). View All» Control: HeLa acid extracts View All» Quality Assurance: Routinely evaluated by immunoblot analysis.Immunoblot Analysis: A 1:1000-1:2000 dilution of this lot detected monomethyl histone H4 (Lys20) in HeLa acid extracts. (Figure A). View All» Presentation: Cultured supernantant in 0.05% sodium azide View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.For maximum recovery of product, centrifuge the vial prior to removing the cap. View All» UniProt Number: P62805 View All» Entrez Gene Number: NM_175054 View All» Gene Symbol:

• HIST1H4A

• H4/J

• HIST1H4F

• HIST1H4L

• H4FN

• H4FA

• H4FH

• HIST1H4H

• H4F2

• H4FM

• H4/A

• H4FK

• H4FG

• HIST2H4

• H4FB

• H4/K

• HIST1H4I

• H4/N

• HIST1H4B

• H4FE

• H4/M

• H4/a

• HIST1H4E

• HIST4H4

• HIST2H4A

• H4FC

• H4FI

• H4/H

• HIST1H4C

• H4/G

• HIST1H4K

• H4FJ

• H4FD

• H4/I

• H4/B

• H4/D

• H4/C

• HIST1H4J

• HIST1H4D

• H4/E
  View All» Alternate Names:
  • H4K20me1

  • Histone H4 (mono methyl K20)

  • histone 4, H4

  • histone H4

  • histone cluster 4, H4
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Multiplexing
      View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. View All» UniProt Summary: FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus.PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family. View All» Brand Family: Upstate View All» Product Name: Anti-monomethyl-Histone H4 (Lys20), clone NL314 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µl View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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