货号：MAB5406B

品牌：Merck Millipore

规格：100UL

目录价：￥6588.00

市场价格：￥5599.80

会员价格：￥5270.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate | MAB5406B View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Reacts with GAD67. No detectable cross reactivity with GAD65 by Western blot on rat brain lysate. View All» Molecular Weight: The unconjugated parent antibody (Cat. No. MAB5406) has an observed molecular weight at ~67 kDa. View All» Immunogen: Recombinant GAD67 protein View All» Clone: 1G10.2 View All» Isotype: IgG2a View All» Background Information: Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Rat. Predicted to react with Human and Mouse based on 100% sequence homology. View All» Application Notes: Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAD67 in rat E18 primary cortex cells. View All» Control: Rat cortex tissue View All» Quality Assurance: Evaluated by Immunohistochemistry in rat cortex tissue.Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected GAD67 in rat cortex tissue. View All» Purification Method: Protein A purified View All» Presentation: Purified mouse monoclonal IgG2a conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA. View All» Storage Conditions: Maintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt. View All» UniProt Number: Q99259 View All» Entrez Gene Number: NP_000808 View All» Gene Symbol:
  • GAD1

  • GAD

  • GAD67
    View All» Alternate Names:
    • Glutamate decarboxylase 1

    • 67 kDa glutamic acid decarboxylase

    • GAD-67

    • Glutamate decarboxylase 67 kDa isoform
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry View All» UniProt Summary: FUNCTION: Catalyzes the production of GABA.CATALYTIC ACTIVITY: L-glutamate = 4-aminobutanoate + CO2.COFACTOR: Pyridoxal phosphate. SUBUNIT STRUCTURE: Homodimer. TISSUE SPECIFICITY: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. INVOLVEMENT IN DISEASE: Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. SEQUENCE SIMILARITIES: Belongs to the group II decarboxylase family. View All» Product Name: Anti-GAD67, clone 1G10.2, Biotin Conjugate View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Biotin View All» Host: Mouse View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-131987.htm
      © Merck Millipore代理MAB5406B Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate;抗-GAD67抗体，克隆1G10.2，生物素结合物store at +2℃ to +8℃，产品报价联系微信jinshanbio