货号：04-773

品牌：Merck Millipore

规格：100Ul

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19 | 04-773 View All» Replaces: 05-773 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Specificity: Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa. View All» Molecular Weight: Doublet at ~130 kDa View All» Epitope: pThr850(Ch) or pThr853(H) View All» Immunogen: KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1. View All» Modifications: Phosphorylation View All» Clone: SA19 View All» Isotype: IgG View All» Background Information: Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT. View All» Species Reactivity:

• Chicken

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, Chicken.Predicted to cross-react with mouse and rat. View All» Control: HEK293 cell lysate phosphorylated by ROKα/ROCKII. View All» Quality Assurance: Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338). View All» Presentation: Cultured supernantant containing 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: O14974 View All» Entrez Gene Number: NP_002471.1 View All» Gene Symbol:
  • MBS

  • MGC133042

  • MYPT1
    View All» Alternate Names:
    • Myosin phosphatase target subunit 1

    • Myosin phosphatase-targeting subunit 1

    • Protein phosphatase myosin-binding subunit

    • myosin phosphatase, target subunit 1

    • protein phosphatase 1, regulatory (inhibitor) subunit 12A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increasedphosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosinphosphatase through the action of Rho-kinase. Several transcriptvariants encoding different isoforms have been found for this gene. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: Regulates myosin phosphatase activity.SUBUNIT STRUCTURE: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT By similarity. Binds PPP1R12B, ROCK1 and IL16.SUBCELLULAR LOCATION: Cytoplasm. Note: Along actomyosin filaments and stress fibers. Ref.8PTM: Phosphorylated by CIT (Rho-associated kinase) By similarity. Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.SEQUENCE SIMILARITIES: Contains 6 ANK repeats. View All» Product Name: Anti-phospho-MYPT1 (Thr850), clone SA19 View All» Concentration: Cultured supernantant containing 0.05% sodium azide. View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μl View All» Host: Rabbit View All»
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