货号：04-857

品牌：Merck Millipore

规格：100UL

目录价：￥4967.00

市场价格：￥4221.95

会员价格：￥3973.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 | 04-857 View All» Replaces: 05-857 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: phospho-c-Src (Tyr416) View All» Molecular Weight: 60kDa View All» Immunogen: KLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src View All» Modifications: Phosphorylation View All» Clone: 2N8 View All» Isotype: IgG View All» Background Information: Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated. View All» Species Reactivity:

• Vertebrates

• Mouse
  View All» Species Reactivity Note: Mouse. Broad species cross-reactivity expected based on sequence homology. View All» Application Notes: Western Blot Analysis: A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A). Immunoprecipitation: A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B). View All» Control: RIPA cell lysate View All» Quality Assurance: Routinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) View All» Presentation: Cultured supernantant in 0.05% sodium azide View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P12931 View All» Entrez Gene Number: NM_005417 View All» Gene Symbol:
  • pp60c-src

  • ASV

  • c-SRC

  • SRC1

  • p60-Src

  • c-src

  • SRC
    View All» Alternate Names: KAT13A View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. View All» UniProt Summary: SIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 | 04-857 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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