货号：05-1044

品牌：Merck Millipore

规格：100Ug

目录价：￥4438.00

市场价格：￥3772.30

会员价格：￥3550.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Sin1 Antibody, clone 1C7.2 | 05-1044 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Detects Sin-1 and its isoforms. View All» Molecular Weight: Sin1 is 59 kDa. Isoforms are 36, 37, 41, 53, 54, and 55 kDa. View All» Immunogen: Full-length Human Sin1 GST-fusion protein. View All» Clone: 1C7.2 View All» Isotype: IgG2aκ View All» Background Information: Sin1 (stress-activated protein kinase (SAPK)-interacting protein 1, MAPKAP1) is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase along with Sin1, GL (mLST8), Protor1, Protor2, and Rictor. The complex that is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Sin1 is also known to directly interact without proteins that include Ras, SPAKs, and JNK.Sin1 is known to have multiple alternative splicing isoforms. Full-length Sin1 is a 522aa protein (59kDa). Sin1 (Isoform 5) (36 kDa) has exon 6 spliced to an alternative exon 7a that results in the loss of both the RBD and PHL domains. Sin1Isoform 2) (55 kDa) lacks exon 7 and lacks the RBD. Sin1 (Isoform 3) (53 kDa) lacks exon 8 lacks the PHL domain. Sin1 (Isoform 4) (37 kDa) lacks exon 1. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, Mouse, and Rat. Other species have not been tested. View All» Quality Assurance: Western Blot Analysis: This lot detected Sin1 at 1:1,000 dilution in A431 cell lysate resolved via SDS-PAGE and transferred to PVDF. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% NaN3. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. View All» UniProt Number: Q9BPZ7 View All» Entrez Gene Number: NP_001006618 View All» Gene Symbol:
  • JC310

  • MGC2745

  • MIP1

  • SIN1
    View All» Alternate Names:
    • MEKK2-interacting protein 1

    • Mitogen-activated protein kinase 2-associated protein 1

    • SAPK-interacting protein 1

    • Stress-activated map kinase-interacting protein 1

    • TORC2 subunit MAPKAP1

    • mitogen-activated protein kinase associated protein 1

    • ras inhibitor MGC2745

    • stress-activated map kinase interacting protein 1

    • stress-activated protein kinase-interacting 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunohistochemistry (Paraffin)

      • Immunofluorescence
        View All» Entrez Gene Summary: This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq] View All» UniProt Summary: Function: Essential component of the TORC2 complex, which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Ref.2 Ref.9 Ref.12 Ref.13 Ref.14Subunit structure: All isoforms except isoform 4 are able to be part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2. Ref.2 Ref.12 Ref.14Subcellular location: Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus. Ref.12 Ref.14Tissue specificity: Ubiquitously expressed, with highest levels in heart and skeletal muscle. Ref.2Sequence similarities: Belongs to the SIN1 family. View All» Product Name: Anti-Sin1, clone 1C7.2 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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