货号：05-1056

品牌：Merck Millipore

规格：EA

目录价：￥3372.00

市场价格：￥2866.20

会员价格：￥2697.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-PTEN (Ser380) Antibody, clone 5.15.12 | 05-1056 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody detects PTEN when phosphorylated on Ser380. View All» Molecular Weight: 55 kDa View All» Epitope: Phosphorylated Ser380 (C-terminus) View All» Immunogen: Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser380 of human PTEN. View All» Modifications: Phosphorylation View All» Clone: 5.15.12 View All» Isotype: IgG View All» Background Information: The protein and lipid phosphatase PTEN (phosphatase and tensin homolog deleted on chromosome ten) has been suggested to play a role in tumorgenesis. PTEN is a tumor suppressor whose loss permits constitutive signaling through the PI3K pathway and is sufficient to cause cancer. In normal cells, FOXO1A and PTEN function together to restrain cell growth. PTEN metabolizes PI(3,4,5)P3, the product of PI3K phosphorylation of PI(4,5)P2 following receptor activation. In cells lacking PTEN, there is an elevated level of PI(3,4,5)P3, which acts as a potent second messenger promoting oncogenesis. Restoration of PTEN function is considered a novel approach to cancer therapy. Overexpression of PTEN inhibits cell migration, and expression of antisense PTEN mRNA enhances migration. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Vertebrates
  View All» Species Reactivity Note: Human, Mouse, and Rat. Predicted to cross-react with many other species based on 100% sequence homology with immunogen. View All» Quality Assurance: Evaluated by western blot on NGF-treated PC12 cell lysate.Western Blot Analysis: 1:5,000 dilution of this antibody was used to detect PTEN phosphorylated on Ser380 in NGF-treated PC12 cell lysate. View All» Presentation: Rabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» UniProt Number: P60484 View All» Entrez Gene Number: NP_000305.3 View All» Gene Symbol:
  • PTEN1

  • TEP1

  • MMAC1

  • MHAM

  • BZS
    View All» Alternate Names:
    • MMAC1 phosphatase and tensin homolog deleted on chromosome

    • Mutated in multiple advanced cancers 1

    • phosphatase and tensin homolog
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq] View All» UniProt Summary: FUNCTION: Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. CATALYTIC ACTIVITY: Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 4,5-bisphosphate + phosphate. A phosphoprotein + H2O = a protein + phosphate. Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. COFACTOR: Magnesium.SUBUNIT STRUCTURE: Monomer. The unphosphorylated form interacts with the second PDZ domain of AIP1 and with DLG1 and MAST2 in vitro. Interacts with MAGI2, MAGI3, MAST1 and MAST3, but neither with MAST4 nor with DLG5. Interaction with MAGI2 increases protein stability. SUBCELLULAR LOCATION: Cytoplasm. Ref.1TISSUE SPECIFICITY: Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas. INDUCTION: Down-regulated by transforming growth factor beta (TGF-beta). Ref.1DOMAIN: The C2 domain binds phospholipid membranes in vitro in a Ca2+-independent manner; this binding is important for its tumor suppressor function. Ref.14 Ref.21PTM: Phosphorylated in vitro by MAST1, MAST2 and MAST3. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. INVOLVEMENT IN DISEASE: Mutations of PTEN are found in a large number of cancers.SEQUENCE SIMILARITIES: Contains 1 C2 tensin-type domain.Contains 1 phosphatase tensin-type domain. View All» Product Name: Anti-phospho-PTEN (Ser380) Antibody, clone 5.15.12 | 05-1056 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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