货号:MAB8140
品牌:Merck Millipore
规格:100Ug
目录价:¥3365.00
市场价格:¥2860.25
会员价格:¥2692.00
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Description:
Anti-Cytomegalovirus Antibody, early, clone 5A8.2 | MAB8140
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Trade Name:
Chemicon (Millipore)
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Specificity:
Reacts with Immediate Early 2 protein (IE2) and its fragments. Recognizes a can epitope near the carboxy-end that includes an epitope found in IE2 86, IE2 60 and IE2 40. In western blot recognizes proteins at apparent molecular weights of 86, 68-72, 55 and 38 kD. Can detect CMV infection 1 hour post-infection exhibiting nuclear and/or intranuclear inclusion staining with reaches peak intensity at 3 hours.With CMV the antigens expressed at different times are listed as: Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD. Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis. Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
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Epitope:
early
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Immunogen:
Affinity purified antigen from MRC-5 cells infected with CMV AD169 (ATCC).
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Clone:
5A8.2
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Isotype:
IgG1
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Species Reactivity:
Human
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Application Notes:
Immunochemistry. IFA at 1:3,000-1:6,000 on acetone fixed cells. Does not work with paraffin embedded tissue sections. Dilute with buffer pH 7.4-7.6 to desired working volume. For extensive dilution, protein containing or other stabilizing medium should be used. Final working dilutions must be determined by end user.
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Presentation:
Purified ascites. Supplied in PBS buffer, pH 7.4. Contains 0.01% sodium azide and carrier protein. Has been sterile filtered.
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Storage Conditions:
Maintain at +4°C for up to 12 months
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Alternate Names:
CMV
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Immunofluorescence
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Western Blotting
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Immunohistochemistry
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Immunoprecipitation
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Brand Family:
Chemicon
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Product Name:
Anti-Cytomegalovirus Antibody, early, clone 5A8.2 | MAB8140
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Antibody Type:
Monoclonal Antibody
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Qty/Pk:
100 µg
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Format:
Purified
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Host:
Mouse
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