货号：05-1070

品牌：Merck Millipore

规格：

目录价：￥3346.00

市场价格：￥2844.10

会员价格：￥2676.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PRAS40 Antibody, clone 9D10.2 | 05-1070 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes PRAS40. View All» Molecular Weight: ~ 40 kDa View All» Epitope: N/A View All» Immunogen: Recombinant protein corresponding to human PRAS40. View All» Clone: 9D10.2 View All» Isotype: IgG2bκ View All» Background Information: PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate, as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to, and regulate mTORC1 (mTOR, Raptor, mLST8) kinase activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it to bind Raptor of mTORC1 in the absence of insulin1. It is thought that PRAS40 inhibits the kinase activity of mTORC1, when bound to Raptor, by preventing binding to its substrates such as p70S6K and 4EBP1. View All» Species Reactivity:

• Mouse

• Human

• Rat
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. View All» Application Notes: Western Blot Analysis: 1 µg/mL from a previous lot detected PRAS40 on 10 µg of HeLa and PC12 cell lysate.Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated PRAS40 from 100 µg of NIH/3T3 cells.Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected PRAS40 in NIH/3T3 cells. View All» Control: NIH/3T3 cell lysate View All» Quality Assurance: Evaluated by Western Blot in NIH/3T3 cell lysate.Western Blot Analysis: 0.1 µg/mL of this antibody detected PRAS40 on 10 µg of NIH/3T3 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q96B36 View All» Entrez Gene Number: NP_001092102.1 View All» Gene Symbol:
  • AKT1S1

  • Lobe

  • PRAS40
    View All» Alternate Names:
    • 40 kDa proline-rich AKT substrate

    • AKT1 substrate 1 (proline-rich)

    • proline-rich Akt substrate, 40 kDa
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunocytochemistry
        View All» Entrez Gene Summary: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]. View All» UniProt Summary: FUNCTION: May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.SIZE: 256 amino acids; 27383 Da SUBUNIT: The phosphorylated form interacts with 14-3-3.SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity).TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293). View All» Product Name: Anti-PRAS40, clone 9D10.2 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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