货号：05-1140

品牌：Merck Millipore

规格：1EA

目录价：￥5181.05

市场价格：￥4403.89

会员价格：￥4144.84

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18 | 05-1140 View All» Replaces: MAB1144 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Reacts specifically with Focal Adhesion Kinase (FAK) when phosphorylated on Tyr397. FAK is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the "Focal Adhesion Targeting" sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation at Tyr-397, and activation of FAK through phosphorylation of Tyr residues (Tyr-576 and Tyr577) in the kinase domain activation loop. View All» Molecular Weight: 125 kDa View All» Epitope: Tyrosine 397 View All» Immunogen: Generated from human FAK, a.a. 393-404, phosphorylated on Tyr397. View All» Modifications: Phosphorylation View All» Clone: 18 View All» Isotype: IgG1 View All» Background Information: FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. Tyrosine 397 is the autophosphorylation site of Focal Adhesion Kinase. The site binds Src family SH2 domains and the p85 subunit of PI3-Kinase. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Proven to react with human and mouse. View All» Control: LPS treated RAW 264 lysates. View All» Quality Assurance: Evaluated by Western Blot in LPS treated RAW 264 lysates.Western Blot Analysis: 1:500 dilution of this lot detected phospho-FAK (Tyr397) on 10 ug of LPS treated RAW 264 lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1 in aqueous buffered solution containing 50% glycerol, BSA, and View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q05397 View All» Entrez Gene Number: Q05397 View All» Gene Symbol:
  • EC 2.7.10.2

  • FADK

  • FAK

  • FAK1

  • pp125FAK
    View All» Alternate Names:
    • FADK 1

    • PTK2 protein tyrosine kinase 2

    • Protein-tyrosine kinase 2

    • focal adhesion kinase 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1 and STEAP4. SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions.TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity.The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.PTM: Phosphorylated on 6 tyrosine residues upon activation. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.Contains 1 FERM domain.Contains 1 protein kinase domain. View All» Product Name: Anti-phospho-Focal Adhesion Kinase (Tyr397), clone 18 View All» Concentration: 0.25 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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