货号：05-1154

品牌：Merck Millipore

规格：1EA

目录价：￥4170.83

市场价格：￥3545.21

会员价格：￥3336.66

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-JAK1 Antibody, clone 73 | 05-1154 View All» Replaces: 05-1154 is a recommended replacement for MAB3700 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Recognizes JAK1 (Janus Kinase 1). The members of the JAK family of cytoplasmic protein tyrosine kinases physically associate with ligand-bound receptors. It is this association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members. View All» Molecular Weight: 130 kDa View All» Immunogen: Generated from human JAK1, amino acids 551-776. View All» Clone: 73 View All» Isotype: IgG2b View All» Background Information: JAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. They regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs (Signal Transducers and Activators of Transcription), which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal STAT transcription factors, which subsequently dimerize and translocate to the nucleus where they bind DNA. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 (Tyr1007/1008) are sites of trans- or autophosphorylation in vivo and in vitro kinase reactions. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. JAK3 is required for signaling through cytokine receptors which share a common gamma-chain. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Canine

• Xenopus

• Chicken
  View All» Species Reactivity Note: Human, mouse, rat, canine, chick, and Xenopus View All» Application Notes: Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation under native and denatured conditions.Immunofluorescence: A previous lot of this antibody was used in IF.Optimal working dilutions must be determined by end user. View All» Control: Huvec cell lysate, whole Jurkat cell lysate View All» Quality Assurance: Routinely evaluated by Western Blot on Huvec lysates.Western Blot Analysis: 1:500 dilution of this lot detected JAK1 on 10 μg of Huvec lysates. View All» Purification Method: Purified View All» Presentation: Purified mouse monoclonal IgG2b aqueous buffered solution containing 50% glycerol, BSA, and View All» Storage Conditions: Stable frozen at -20°C in undiluted aliquots for up to 1 year from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P23458 View All» Entrez Gene Number: NM_002227.2 View All» Gene Symbol:
  • JAK1

  • JAK1B

  • JAK-1

  • JAK1A

  • EC 2.7.10.2
    View All» Alternate Names:
    • Janus kinase 1

    • Janus kinase 1 (a protein tyrosine kinase) 1

    • Tyrosine-protein kinase JAK1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunofluorescence
        View All» Entrez Gene Summary: Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. View All» UniProt Summary: FUNCTION: SwissProt: P23458 # Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.SIZE: 1142 amino acids; 131957 Da SUBUNIT: Interacts with IL31RA and SHB.SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein. Note=Wholly intracellular, possibly membrane associated.DOMAIN: SwissProt: P23458 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. View All» Product Name: Anti-JAK1 Antibody, clone 73 | 05-1154 View All» Concentration: 0.25 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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