货号：05-1248

品牌：Merck Millipore

规格：100Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 | 05-1248 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3. View All» Molecular Weight: ~17 kDa View All» Epitope: monomethylated H3 Lys9 View All» Immunogen: KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3. View All» Clone: CMA306 View All» Isotype: IgG2a View All» Background Information: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Application Notes: Dot Blot Analysis: AbSurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides. View All» Control: Recombinant Histone H3 and HeLa acid extract. View All» Quality Assurance: Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide. View All» Storage Conditions: Stable at 2-8°C for 1 year from date of receipt. View All» UniProt Number: O43463 View All» Entrez Gene Number: NP_003484 View All» Gene Symbol:

• EC 2.1.1.43

• KMT1A

• MG44

• OTTHUMP00000024298

• SUV39H
  View All» Alternate Names:
  • H3K9me1

  • Histone H3 (mono methyl K9)

  • H3-K9-HMTase 1

  • Histone H3-K9 methyltransferase 1

  • Lysine N-methyltransferase 1A

  • Position-effect variegation 3-9 homolog

  • Su(var)3-9 homolog 1

  • Suppressor of variegation 3-9 homolog 1

  • histone-lysine N-methyltransferase, H3 lysine-9 specific 1

  • suppressor of variegation 3-9 (Drosophila) homolog 1

  • suppressor of variegation 3-9 homolog 1 (Drosophila)
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Peptide Inhibition Assay
      View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1,with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. View All» UniProt Summary: FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.7ENZYME REGULATION: Inhibited by S-adenosyl-L-homocysteine. Ref.20SUBUNIT STRUCTURE: Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR. Ref.22 Ref.1 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.16 Ref.17 Ref.18 Ref.24 Ref.25SUBCELLULAR LOCATION: Nucleus. Centromere. Note: Associates with centromeric constitutive heterochromatin. Ref.6DEVELOPMENTAL STAGE: Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.DOMAIN: Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin. Ref.17PTM: Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function. Ref.6 Ref.21 Ref.26 Ref.27SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.Contains 1 chromo domain.Contains 1 post-SET domain.Contains 1 pre-SET domain.Contains 1 SET domain. View All» Product Name: Anti-monomethyl Histone H3 (Lys9), clone CMA306 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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