货号：05-1307

品牌：Merck Millipore

规格：1EA

目录价：￥6376.59

市场价格：￥5420.10

会员价格：￥5101.27

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2 | 05-1307 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Product Overview: DI-UBIQUITIN PROTEIN AVAILABLE!Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408) View All» Specificity: Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin. View All» Molecular Weight: varies View All» Epitope: Lys48 ubiquitination site View All» Immunogen: Immunogen used was human di-ubiquitin linked at Lys48. View All» Clone: Apu2 View All» Isotype: IgG View All» Background Information: Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Rabbit
  View All» Species Reactivity Note: Human, mouse, rat, rabbit. Based on sequence homology, broad species cross-reactivity is expected. View All» Application Notes: Immunohistochemistry: Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.Immunocytochemistry: Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).Flow cytometry: Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red). Immunoprecipitation: Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.Inhibition Assay: Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots. View All» Control: Lys48 polyubiquitin chains. View All» Quality Assurance: Western Blot Analysis: A 1:1000-1:5000 dilution of this lot detected Lys-48 ubiquitin chains by Western blotting. View All» Purification Method: Protein A purfied View All» Presentation: Protein A purified recombinant purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.For maximum recovery of product, centrifuge the vial prior to removing the cap. View All» UniProt Number: P0CG47 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Flow Cytometry

  • Immunocytochemistry

  • Immunoprecipitation

  • Immunohistochemistry

  • Western Blotting

  • Cell Function Assay
    View All» UniProt Summary: FUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21SUBCELLULAR LOCATION: Cytoplasm. Nucleus. PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).SIMILARITY: Belongs to the ubiquitin family. View All» Product Name: Anti-Ubiquitin, Lys48-Specific, clone Apu2 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Purified View All» Host: Rabbit View All»
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