货号：05-1308

品牌：Merck Millipore

规格：100UL

目录价：￥7049.00

市场价格：￥5991.65

会员价格：￥5639.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3 | 05-1308 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Product Overview: DI-UBIQUITIN PROTEIN AVAILABLE!Di-Ubiquitin (Lys63-linked) Recombinant Protein (17-10388) View All» Specificity: Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin. View All» Molecular Weight: varies View All» Epitope: Lys63 ubiquitination site View All» Immunogen: Immunogen used was human di-ubiquitin linked at Lys63. View All» Clone: Apu3 View All» Isotype: IgG View All» Background Information: Polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.In contrast, polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Rabbit
  View All» Species Reactivity Note: Human, mouse, rabbit, rat. Based on sequence homology, broad species cross-reactivity is expected. View All» Application Notes: Immmunoprecipitation: Rabbit monoclonal anti-ubiquitin (Lys-63-specific), clone Apu3, precipitated ubiquitin-containing proteins from HeLa cell lysatesImmunohistochemistry: Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Flow cytometry: Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys63-specific (#05-1308) (blue) and rabbit IgG control (red). Inhibition Assay: Preincubation of anti-ubiquitin, Lys63-specific, with K63-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.Immunocytochemistry: Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat, and rabbit brain sections. View All» Control: Lys63 polyubiquitin chains. View All» Quality Assurance: Western Blot Analysis: A 1:1000 dilution of this antibody detected Lys-63 ubiquitin chains by Western blotting, but did not detect free ubiquitin or Lys48 polyubiquitin chains. View All» Purification Method: Protein A purfied View All» Presentation: Protein A purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.For maximum recovery of product, centrifuge the vial prior to removing the cap. View All» UniProt Number: P0CG47 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Cell Function Assay

  • Immunocytochemistry

  • Immunoprecipitation

  • Immunohistochemistry

  • Western Blotting
    View All» UniProt Summary: FUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21SUBCELLULAR LOCATION: Cytoplasm. Nucleus. PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).SIMILARITY: Belongs to the ubiquitin family. View All» Product Name: Anti-Ubiquitin, Lys63-Specific, clone Apu3 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Purified View All» Host: Rabbit View All»
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