货号：05-1327

品牌：Merck Millipore

规格：100Ug

目录价：￥4439.00

市场价格：￥3773.15

会员价格：￥3551.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PHD2 Antibody, clone 76a | 05-1327 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes PHD2. View All» Molecular Weight: ~ 46 kDa View All» Epitope: Unknown View All» Immunogen: Recombinant protein corresponding to human PHD2. View All» Clone: 76a View All» Isotype: IgG1κ View All» Background Information: PHD2 (Prolyl hydroxylase domain-containing protein 2), also known as Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2 or HPH-2), is one of 4 PHD (PHD1-4) proteins which function as prolyl hydroxylases to effect oxygen homeostasis via hypoxia-inducible factor (HIF). This transcriptional complex is composed of an alpha-beta heterodimer that mediates a broad range of cellular and systemic responses to hypoxia. The PHD proteins hydroxylate HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. The PHD2 isoform is the main down-regulator of HIFs for normal oxygen levels and mild hypoxia. When oxygen levels are normal, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha. This post-translational modification targets this protein for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Control: MCF-7 cell lysate View All» Quality Assurance: Evaluated by Western Blot in MCF-7 cell lysate.Western Blot Analysis: 2 µg/ml of this antibody detected PHD2 on 10 µg of MCF-7 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q9GZT9 View All» Entrez Gene Number: NP_071334.1 View All» Gene Symbol:

• EGLN1

• C1orf12

• ECYT3

• HIF-PH2

• HIFPH2

• HPH-2

• HPH2

• PHD2

• SM-20

• SM20

• ZMYND6
  View All» Alternate Names:
  • EGL nine (C.elegans) homolog 1

  • HIF prolyl hydroxylase 2

  • HIF-prolyl hydroxylase 2

  • Hypoxia-inducible factor prolyl hydroxylase 2

  • Prolyl hydroxylase domain-containing protein 2

  • egl nine homolog 1

  • egl nine homolog 1 (C. elegans)

  • egl nine-like protein 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. CATALYTIC ACTIVITY: An HIF alpha chain L-proline + 2-oxoglutarate + O2 = An HIF alpha chain trans-4-hydroxy-L-proline + succinate + CO2.COFACTOR: Binds 1 Fe2+ ion per subunit.Ascorbate.ENZYME REGULATION: Following exposure to hypoxia, activated in HeLa cells but not in cardiovascular cells. Seems to be inhibited by ING4. SUBUNIT STRUCTURE: Monomer. Interacts with ING4. TISSUE SPECIFICITY: According to Ref.1 widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to Ref.8 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. INVOLVEMENT IN DISEASE: Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels. SEQUENCE SIMILARITIES: Contains 1 MYND-type zinc finger.Contains 1 PKHD (prolyl/lysyl hydroxylase) domain.CAUTION: It was previously reported that this protein was the ortholog of rat SM-20. However, EGLN3 is now considered the true ortholog of rat SM-20 since it shows substantially greater similarity.SEQUENCE CAUTION: The sequence AAK07536.1 differs from that shown. Reason: Frameshift at position 239. View All» Product Name: Anti-PHD2, clone 76a View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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