货号：05-1459

品牌：Merck Millipore

规格：200Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-SMAD1 Antibody, clone AS22 | 05-1459 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Detects SMAD1 View All» Molecular Weight: 52 kDa View All» Immunogen: GST-fusion protein corresponding to amino acids 1-110 of human SMAD1. View All» Clone: AS22 View All» Isotype: IgG1κ View All» Background Information: Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human and Mouse. Other species were not tested. View All» Application Notes: Western Blot Analysis: This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF. View All» Quality Assurance: Routinely evaluated by Western BlotWestern Blot Analysis: This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. View All» UniProt Number: Q15797 View All» Entrez Gene Number: NP_001003688 View All» Gene Symbol:
  • BSP-1

  • BSP1

  • CMT2D

  • GARS

  • HMN5

  • JV4-1

  • JV41

  • MADH1

  • MADR1

  • hSMAD1
    View All» Alternate Names:
    • SMAD 1

    • SMAD family member 1

    • SMAD, mothers against DPP homolog 1

    • MAD, mothers against decapentaplegic homolog 1

    • Mad-related protein 1

    • Mothers against DPP homolog 1

    • Sma- and Mad-related protein 1

    • TGF-beta signaling protein 1

    • Transforming growth factor-beta-signaling protein 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunofluorescence
        View All» Entrez Gene Summary: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq] View All» UniProt Summary: FUNCTION: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SUBUNIT: Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. TISSUE SPECIFICITY: Ubiquitous. Highest expression seen in the heart and skeletal muscle. PTM: Phosphorylated on serine by BMP type 1 receptor kinase. PTM: Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. View All» Product Name: Anti-SMAD1, clone AS22 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Mouse View All»
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