货号：05-1510

品牌：Merck Millipore

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目录价：￥3908.00

市场价格：￥3321.80

会员价格：￥3126.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Aly/REF Antibody, clone 11G5 | 05-1510 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Aly/REF. View All» Molecular Weight: ~ 32 kDa View All» Epitope: Unknown View All» Immunogen: Recombinant protein corresponding to human Aly/REF. View All» Clone: 11G5 View All» Isotype: IgG1κ View All» Background Information: Aly (THO complex subunit 4 or THOC4 or Aly/REF) is a heat stable, nuclear protein that functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. Aly (THOC4) is also preferentially associated with intronless mRNAs in the nucleus. Loading of export factors onto mRNAs is a key step in gene expression. In vertebrates, splicing plays a role in this process. Specific protein complexes, exon junction complexes and transcription/export complexes, are loaded onto mRNAs in a splicing-dependent manner, and adaptor proteins such as Aly (THOC4) in the complexes in turn recruit mRNA exporters onto the RNA. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Application Notes: Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Aly/REF in NIH/3T3 and HeLa cells.Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Kim, V.N., et al. (2001). The EMBO Journal. 20, 2062-2068.) View All» Control: Hek293 cell lysate View All» Quality Assurance: Evaluated by Western Blot in Hek293 cell lysate.Western Blot Analysis: 0.5 µg/ml of this antibody detected Aly/REF on 10 µg of Hek293 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q86V81 View All» Entrez Gene Number: NP_005773.3 View All» Gene Symbol:

• ALY

• BEF

• Tho4

• THOC4
  View All» Alternate Names:
  • Ally of AML-1 and LEF-1

  • THO complex 4

  • Transcriptional coactivator Aly/REF

  • bZIP enhancing factor

  • bZIP-enhancing factor BEF

  • transcriptional coactivator
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry

    • Immunoprecipitation

    • Immunohistochemistry
      View All» Entrez Gene Summary: The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Directs mRNA derived from Herpes simplex virus intron-less genes to the NXF1-mediated export pathway.SUBUNIT STRUCTURE: Homomultimer. Binds LEF1 and RUNX1 By similarity. Is part of several complexes involved in mRNA processing and export. Part of the heteromultimeric THO/TREX complex containing THOC1, THOC2, THOC3, THOC4 and UAP56. Associates with the spliceosome. Binds NXF1 and RBM8A directly and is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RBM8A, SRRM1, NXF1, RENT2, RENT3B and THOC4 By similarity. Found in a mRNA splicing-dependent exon junction complex (EJC) with DEK, RBM8A, RNPS1, SRRM1 and THOC4. Binds UL54 from Herpes simplex type 1 (strain KOS). Found in a mRNP complex with RENT3A and RENT3B. Interacts with BAT1, RBM8A, RNPS1 and SRRM1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. PTM: Arg-50 and Arg-204 are dimethylated, probably to asymmetric dimethylarginine. Phosphorylated upon DNA damage, probably by ATM or ATR. INVOLVEMENT IN DISEASE: Antibodies against THOC4 are found in sera of patients with systemic lupus erythematosus (SLE). SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain. View All» Product Name: Anti-Aly/REF, clone 11G5 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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