货号：05-1526

品牌：Merck Millipore

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目录价：￥3789.00

市场价格：￥3220.65

会员价格：￥3031.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Importin subunit alpha-1 Antibody, clone 8G5 | 05-1526 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Importin subunit alpha-1. View All» Molecular Weight: ~ 60 kDa View All» Epitope: Unknown View All» Immunogen: Recombinant protein corresponding to human Importin subunit alpha-1. View All» Clone: 8G5 View All» Isotype: IgG1κ View All» Background Information: The importin alpha/beta heterodimer facilitates the translocation of hundreds of proteins across the nuclear envelope. Importin alpha binds to proteins containing a classical nuclear localization signal and links them to importin beta ((KPNB1), a protein which ferries the complex through the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Control: K562 cell lysate View All» Quality Assurance: Evaluated by Western Blot in K562 cell lysate.Western Blot Analysis: 0.01 µg/ml of this antibody detected Importin subunit alpha-1 on 10 µg of K562 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P52294 View All» Entrez Gene Number: NP_002255.3 View All» Gene Symbol:

• RCH2

• NPI-1

• SRP1

• SRP1-beta

• IPOA5

• KPNA1
  View All» Alternate Names:
  • karyopherin alpha 1 (importin alpha 5)

  • importin alpha 5

  • importin-alpha-S1

  • recombination activating gene cohort 2

  • Karyopherin subunit alpha-1

  • Nucleoprotein interactor 1

  • RAG cohort protein 2

  • karyopherin alpha 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Two transcript variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. SUBUNIT STRUCTURE: Forms a complex with importin subunit beta-1. Interacts with ANP32E by similarity. Interacts with the nucleoprotein of influenza A viruses. Binds to HCMV (human cytomegalovirus) UL84, HIV-1 Vpr and to ebolavirus VP24. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Expressed ubiquitously.DOMAIN: Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import. The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins by similarity. The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding by similarity. SEQUENCE SIMILARITIES: Belongs to the importin alpha family.Contains 10 ARM repeats.Contains 1 IBB domain. View All» Product Name: Anti-Importin subunit alpha-1, clone 8G5 Antibody (05-1526) View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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