货号：05-154

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PKCα Antibody, clone M4 | 05-154 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Specific for PKCα. MW: ~82 kDa. View All» Molecular Weight: ~82 kDa View All» Immunogen: PKCα purified from rabbit brain cytosol. View All» Clone: M4 View All» Isotype: IgG1 View All» Background Information: Protein Kinase c alpha (PKC alpha) is a 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell-cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy-terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation. View All» Species Reactivity:

• Human

• Bovine

• Mouse

• Rat

• Rabbit
  View All» Species Reactivity Note: Bovine, human, mouse, rat and rabbit. View All» Application Notes: Immunoprecipitation: 4 μg of a previous lot immunoprecipitated PKCα from an A431 RIPA cell lysate and a bovine brain extract.Neutralization: An independent laboratory showed that 20 μg of this antibody can inhibit >50% of the kinase activity of 20 ng PKCα (Leach, K.L., 1998). View All» Control: Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. View All» Quality Assurance: Evaluated in A431 RIPA cell lysate or bovine brain cytosolic extract.Western Blot Analysis: 0.5-2 μg/mL of this antibody detected PKCα from a human A431 RIPA cell lysate. Previous lots detected PKCα in bovine brain cytosolic extract. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG1 in storage buffer containing (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» UniProt Number: P17252 View All» Entrez Gene Number: NM_002737 View All» Gene Symbol:
  • PRKCA

  • PKC-A

  • AAG6

  • PKC-alpha

  • MGC129901

  • MGC129900

  • PRKACA

  • PKCA
    View All» Alternate Names:
    • aging-associated gene 6

    • protein kinase C alpha type

    • protein kinase C, alpha
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Neutralizing

      • Radioimmunoassay

      • Western Blotting
        View All» Entrez Gene Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. View All» UniProt Summary: FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).SIZE: 672 amino acids; 76764 Da SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-PKCα Antibody, clone M4 | 05-154 View All» Concentration: 0.95 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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