货号：05-157

品牌：Merck Millipore

规格：200Ug

目录价：￥5513.00

市场价格：￥4686.05

会员价格：￥4410.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 | 05-157 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes MAP kinase encoded by the mapk gene, Mr 42 kDa. View All» Molecular Weight: 42 kDa View All» Immunogen: HPLC purified murine recombinant MAP Kinase. Clone 1B3B9 View All» Clone: 1B3B9 View All» Isotype: IgG2a View All» Background Information: ERK2 is activated by MEK2, which phosphorylates neighboring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transeitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus. View All» Species Reactivity:

• Avian

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Proven to react with Human, Mouse, and Rat. Other species cross reactivity is unknown. View All» Application Notes: Immunoprecipitation: 4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase. View All» Control: A431, HeLa and NIH/3T3 cell lysatesIncluded Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated in RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts, or rat L6 cells.Western Blot Analysis: 0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells. View All» Purification Method: Protein G chromatography View All» Presentation: Purified mouse monoclonal IgG2a in buffer containing PBS with 0.05% sodium azide. Store frozen at -20°C, avoid freeze thaw cycles. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P28482 View All» Entrez Gene Number: NM_002745 View All» Gene Symbol:
  • MAPK1

  • p38

  • ERK

  • P42MAPK

  • ERK2

  • p40

  • PRKM1

  • p41mapk

  • PRKM2

  • MAPK2

  • p42-MAPK

  • p41

  • ERK-2

  • ERT1
    View All» Alternate Names:
    • Extracellular signal-regulated kinase 2

    • MAP kinase 2

    • MAPK 2

    • Mitogen-activated protein kinase 2

    • extracellular signal-regulated kinase-2

    • mitogen-activated protein kinase 1

    • protein tyrosine kinase ERK2.
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).COFACTOR: Magnesium (By similarity).SIZE: 360 amino acids; 41390 Da SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain View All» Brand Family: Upstate View All» Product Name: Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 | 05-157 View All» Concentration: 0.76 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Mouse View All»
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