货号：MABC175

品牌：Merck Millipore

规格：

目录价：￥4118.00

市场价格：￥3500.30

会员价格：￥3294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-LC3A Antibody, clone EPR1754, Rabbit Monoclonal | MABC175 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the N-terminus of LC3A. View All» Molecular Weight: ~16 kDa View All» Immunogen: Synthetic peptide corresponding to the N-terminus of human LC3A. View All» Clone: EPR1754 View All» Background Information: Microtubule-associated LC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Rat, and Mouse. Species cross reactivity is based on WB analysis. View All» Application Notes: Flow Cytometry Analysis: A 1:40 dilution from a representative lot was used to detect LC3A in FC. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysateWestern Blot Analysis: A 1:500 dilution of this antibody was used to detect LC3A in HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q9H492 View All» Entrez Gene Number: Q9H492 View All» Gene Symbol:
  • MAP1ALC3

  • MAP1BLC3

  • ATG8E

  • LC3

  • LC3A

  • OTTHUMP00000030696

  • OTTHUMP00000030697

  • OTTHUMP00000030698
    View All» Alternate Names:
    • microtubule-associated protein 1 light chain 3 alpha

    • Autophagy-related protein LC3 A

    • Autophagy-related ubiquitin-like modifier LC3 A2

    • MAP1 light chain 3-like protein 1

    • MAP1A/MAP1B LC3 A

    • MAP1A/MAP1B light chain 3 A

    • MAP1A/1B light chain 3 A

    • microtubule-associated proteins 1A/1B light chain 3

    • microtubule-associated proteins 1A/1B light chain 3A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Flow Cytometry
        View All» UniProt Summary: FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes).SUBUNIT STRUCTURE: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity.SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.SEQUENCE SIMILARITIES: Belongs to the MAP1 LC3 family. View All» Product Name: Anti-LC3A, clone EPR1754, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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