货号：MABC176

品牌：Merck Millipore

规格：

目录价：￥4118.00

市场价格：￥3500.30

会员价格：￥3294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-LC3A/B (N-term) Antibody, clone EP1983Y, Rabbit Monoclonal | MABC176 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the N-terminus of LC3A/B. View All» Molecular Weight: ~16 kDa View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to the C-terminus of human LC3A/B. View All» Clone: EP1983Y View All» Background Information: Microtubule-associated LC3A/B constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. LC3A/B is composed of a heavy chain and multiple light-chain subunits. Amino acid sequencing of a 120 kd microtubule-binding and light chain-binding fragment of the heavy chain reveals that light chain 1 binds near the heavy chain N-terminus. These data indicate that the heavy chain and light chain 1 are produced by proteolytic processing of a LC3B polyprotein and form a complex microtubule-binding domain. There are three human orthologs of the rat LC3, named LC3A, LC3B, and LC3C. The three isoforms of human LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. LC3A and LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue; LC3B does not undergo C-terminal cleavage and exists in a single modified form. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Rat, and Mouse. View All» Application Notes: Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used to detect LC3A/B in IC.Immunoprecipitation Analysis: A 1:40 dilution from a representative lot was used to detect LC3A/B in IP.Flow Cytometry Analysis: A 1:40 dilution from a representative lot was used to detect LC3A/B in FC.Immunohistochemistry Analysis: A 1:100-250 dilution from a representative lot was used to detect LC3A/B in paraffin-embedded human breast carcinoma tissue. View All» Control: HeLa cell lysate, and human, mouse, and rat brain. View All» Quality Assurance: Evaluated by Western blotting in HeLa cell lystae, and human, mouse, and rat brain tissue lysates.Western Blot Analysis: A 1:1,000-5,000 dilution of this antibody was used to detect LC3A/B in HeLa cell lysate, and human, mouse, and rat brain tissue lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q9H492 View All» Entrez Gene Number: Q9H492 View All» Gene Symbol:
  • MAP1ALC3

  • MAP1BLC3

  • ATG8E

  • LC3

  • LC3A

  • OTTHUMP00000030696

  • OTTHUMP00000030697

  • OTTHUMP00000030698
    View All» Alternate Names:
    • microtubule-associated protein 1 light chain 3 alpha

    • Autophagy-related protein LC3 A

    • Autophagy-related ubiquitin-like modifier LC3 A2

    • MAP1 light chain 3-like protein 1

    • MAP1A/MAP1B LC3 A

    • MAP1A/MAP1B light chain 3 A

    • MAP1A/1B light chain 3 A

    • microtubule-associated proteins 1A/1B light chain 3

    • microtubule-associated proteins 1A/1B light chain 3A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Flow Cytometry

      • Immunocytochemistry

      • Immunoprecipitation

      • Immunohistochemistry (Paraffin)
        View All» UniProt Summary: FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes).SUBUNIT STRUCTURE: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity.SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.SEQUENCE SIMILARITIES: Belongs to the MAP1 LC3 family. View All» Product Name: Anti-LC3A/B (N-term), clone EP1983Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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