货号：MABD102

品牌：Merck Millipore

规格：100Ug

目录价：￥4192.00

市场价格：￥3563.20

会员价格：￥3353.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Lin28, clone PR3G10 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Molecular Weight: ~28 kDa observed View All» Epitope: Near N-Terminus View All» Immunogen: Ovalbumin-conjugated linear peptide corresponding to human Lin28. View All» Clone: PR3G10 View All» Isotype: IgG1κ View All» Background Information: Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells. View All» Species Reactivity: Human View All» Application Notes: Western Blot Analysis: 1 µg/mL from a representative lot detected Lin28 in human fetal brain tissue lysate.Immunocytochemistry Analysis: A 1:500 dilution (2 µg/mL) from a representative lot detected Lin28 in mESCs. View All» Control: Human placenta tissue lysate View All» Quality Assurance: Evaluated by Western Blot in human placenta tissue lysate.Western Blot Analysis: 1 µg/mL of this antibody detected Lin28 in 10 µg of human placenta tissue lysate. View All» Purification Method: Protein G Purified View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q9H9Z2 View All» Entrez Gene Number: NP_078950 View All» Gene Symbol:

• LIN28A

• CSDD1

• LIN28

• ZCCHC1
  View All» Alternate Names:
  • Protein lin-28 homolog A

  • Lin-28A

  • Zinc finger CCHC domain-containing protein 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry
      View All» UniProt Summary: FUNCTION: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.SUBUNIT STRUCTURE: During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent. Interacts with ZCCHC11/TUT4.SUBCELLULAR LOCATION: Cytoplasm. Nucleus > nucleolus. Note: Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes. Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.TISSUE SPECIFICITY: Expressed in embryonic stem cells (ES cells), placenta and testis. View All» Product Name: Anti-Lin28, clone PR3G10 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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