货号：MABE102

品牌：Merck Millipore

规格：

目录价：￥3609.00

市场价格：￥3067.65

会员价格：￥2887.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-FIH-1 Antibody, clone FIH 162c | MABE102 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~40 kDa observed View All» Immunogen: Full length recombinant protein corresponding to human FIH-1. View All» Clone: 162c View All» Isotype: IgG1κ View All» Background Information: Factor inhibiting HIF-1 (FIH-1) is a transcription factor involved in the regulation of cellular responses to hypoxia. FIH-1 is characterized as a molecular oxygen-dependent dioxygenase which limits the transcriptional activity of HIF. This protein is also implicated in both HIF-dependent and -independent hypoxia responses. Studies have shown that FIH-1 binds to VHL which functions as a corepressor of HIF-1α repression by recruiting histone deacetylases. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. View All» Application Notes: Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in U-2OS cells. (Stolze, I., et al. (2004). The Journal of Biological Chemistry. 279:42719–42725.)Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Stolze, I., et al. (2004). The Journal of Biological Chemistry. 279:42719–42725.) View All» Control: HCT116 cell lysate View All» Quality Assurance: Evaluated by Western Blot in HCT116 cell lysate.Western Blot Analysis: 0.25 µg/mL of this antibody detected FIH-1 on 10 µg of HCT116 cell lysate. View All» Purification Method: Protein G View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q9NWT6 View All» Entrez Gene Number: NP_060372 View All» Gene Symbol:

• FIH1

• FIH-1

• HIF1AN
  View All» Alternate Names:
  • hypoxia inducible factor 1, alpha subunit inhibitor

  • Hypoxia-inducible factor asparagine hydroxylase

  • factor inhibiting HIF1

  • peptide-aspartate beta-dioxygenase

  • hypoxia-inducible factor 1-alpha inhibitor

  • Factor inhibiting HIF-1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunohistochemistry

    • Immunofluorescence
      View All» UniProt Summary: FUNCTION: Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. CATALYTIC ACTIVITY: Peptide L-aspartate + 2-oxoglutarate + O2 = peptide 3-hydroxy-L-aspartate + succinate + CO2. COFACTOR: Iron.SUBUNIT STRUCTURE: Homodimer. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. SUBCELLULAR LOCATION: Nucleus (Potential). SEQUENCE SIMILARITIES: Contains 1 JmjC domain. View All» Product Name: Anti-FIH-1, clone FIH 162c View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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