货号：MABE114

品牌：Merck Millipore

规格：

目录价：￥4739.00

市场价格：￥4028.15

会员价格：￥3791.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-LSD1 Antibody, clone 3D12 | MABE114 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~ 92 kDa Observed View All» Epitope: 0 View All» Immunogen: Full length recombinant protein corresponding to human LSD1. View All» Clone: 3D12 View All» Isotype: IgG1κ View All» Background Information: LSD1 (lysine specific demethylase 1), also known as BHC110, and KDM1, is a histone demethylase that specifically demethylates Lys4 of histone H3. Methylation of Histone H3 Lys4 is a specific tag for epigenetic transcriptional activation, suggesting a role for LSD1 as a corepressor. LSD1 plays a role in a number of chromatin-remodeling pathways and has been linked to pathological processes, such as tumorigenesis. LSD1 acts by oxidizing the substrate FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Control: Human testis tissue lysate View All» Quality Assurance: Evaluated by Western Blot in human testis tissue lysate.Western Blot Analysis: 0.05 µg/mL of this antibody detected LSD1 on 10 µg of human testis tissue lysate. View All» Purification Method: Protein G View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O60341 View All» Entrez Gene Number: NP_001009999 View All» Gene Symbol:

• BHC110

• KDM1

• LSD1
  View All» Alternate Names:
  • BRAF35-HDAC complex protein BHC110

  • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit

  • Flavin-containing amine oxidase domain-containing protein 2

  • amine oxidase (flavin containing) domain 2

  • lysine (K)-specific demethylase 1

  • lysine-specific histone demethylase 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. COFACTOR: FAD. SUBUNIT STRUCTURE: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). SUBCELLULAR LOCATION: Nucleus TISSUE SPECIFICTY: Ubiquitously expressed. DOMAIN: The SWIRN domain may act as an anchor site for a histone tail. SEQUENCE SIMILARITIES: Belongs to the flavin monoamine oxidase family.Contains 1 SWIRM domain.BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:KM=3.0 µM for H3 monomethyl-K4 Ref.13KM=4.2 µM for H3 dimethyl-K4KM=3.9 µM for H3 monomethyl-K4-monomethyl-K9KM=17.5 µM for monomethyl-K4-acetyl-K9SEQUENCE CAUTION: The sequence BAA25527.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. View All» Product Name: Anti-LSD1, clone 3D12 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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