货号：MABE17

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho Sir2/SIRT1 (Ser27) Antibody, clone EP2850Y, Rabbit | MABE17 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Sir2/SIRT1 phosphorylated at Ser27. View All» Molecular Weight: ~110 kDa observed View All» Epitope: Ser27 View All» Immunogen: Phospho-specific peptide corresponding to human Sir2/SIRT1 at Ser27. View All» Modifications: Phosphorylation View All» Clone: EP2850Y View All» Isotype: IgG View All» Background Information: The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2, SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4). View All» Species Reactivity: Human View All» Species Reactivity Note: Proven to react with human. View All» Application Notes: Immunoprecipitation Analysis: A 1:20 dilution from a previous lot detected Sir2/SIRT1 in IP. View All» Control: Calyculin A treated and untreated 293T cell lysates View All» Quality Assurance: Evaluated by Western Blot in Calyculin A treated and untreated 293T cell lysate.Western Blot Analysis: A 1:5,000 – 1:10,000 dilution of this antibody detected Sir2/SIRT1 in Calyculin A treated and untreated 293T cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q96EB6 View All» Entrez Gene Number: NP_036370.2 View All» Gene Symbol:

• sirtuin 1

• SIR2L1

• hSIR2

• hSIRT1
  View All» Alternate Names:
  • SIR2-like protein 1

  • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)

  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation
      View All» UniProt Summary: FUNCTION: NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD+/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR: Binds 1 zinc ion per subunit By similarity.ENZYME REGULATION: Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). RPS19BP1/AROS acts as a positive regulator of deacetylation activity. Negatively regulated by KIAA1967/DBC1. SUBUNIT STRUCTURE: Interacts with TAF1B. Found in a complex with PCAF and MYOD1 By similarity. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Interacts with MLLT7/FOXO4, HES1, HEY2, p53/TP53 and PML. Interacts with RPS19BP1/AROS. Interacts with KIAA1967/DBC1 (via N-terminus).SUBCELLULAR LOCATION: Nucleus › PML body. Note: Recruited to the nuclear bodies via its interaction with PML. TISSUE SPECIFICITY: Widely expressed. MISCELLANEOUS: Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast.SEQUENCE SIMILARITIES: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain.SEQUENCE CAUTIONS: The sequence AAH12499.1 differs from that shown. Reason: Erroneous initiation. View All» Product Name: Anti-phospho Sir2/SIRT1 (Ser27), clone EP2850Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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