货号：MABE195

品牌：Merck Millipore

规格：

目录价：￥3522.00

市场价格：￥2993.70

会员价格：￥2817.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-UBF Antibody, clone EP2741Y, Rabbit | MABE195 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes UBF near the N-terminus. View All» Molecular Weight: ~97/94 kDa observed. Uniprot describes two isoforms at 89 and 85 kDa. View All» Epitope: Near N-terminus View All» Immunogen: Synthetic peptide corresponding to human UBF near the N-terminus. View All» Clone: EP2741Y View All» Isotype: IgG View All» Background Information: The eukaryotic upstream binding factor (UBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor, SL1. UBF binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner (1). Experiments reveal that a cooperative interaction between UBF and SL1 leads to the formation of a new protein-DNA complex at the UCE and core elements which indicate that formation of the UBF-SL1 complex is vital for transcriptional activation by UBF (2). UBF contains six HMG box domains. The contribution of each HMG box motif to its function is different (3). Its believed that HMG motifs are the DNA-binding domains of UBF. Altering one of these boxes, as in the second form of UBF, may alter the functional characteristics of the transcription factor. Thus, the existence of different forms of UBF may have important ramifications for transcription by RNA polymerase I (4). View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with human and mouse. View All» Application Notes: Immunocytochemistry Analysis: 1:100-250 dilution from a representative lot was used in IC. Flow Cytometry Analysis: 1:30 dilution from a representative lot was used in FC.Immunohistochemistry Analysis: 1:100-1:250 dilution from a representative lot detected UBF in human breast carcinoma tissue. View All» Control: HeLa and A431 cell lysates View All» Quality Assurance: Evaluated by Western Blot in HeLa and A431 cell lysates.Western Blot Analysis: 1:1,000-10,000 dilution of this antibody detected UBF in HeLa and A431 cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below-20°C may cause glycerol containing Solutions to become frozen during storage. View All» UniProt Number: P17480 View All» Gene Symbol:
  • UBF

  • NOR-90

  • UBF-1

  • UBF1
    View All» Alternate Names:
    • upstream binding transcription factor, RNA polymerase I

    • Autoantigen NOR-90

    • Upstream-binding factor 1

    • nucleolar transcription factor 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Flow Cytometry

      • Immunohistochemistry (Paraffin)

      • Immunocytochemistry
        View All» UniProt Summary: FUNCTION: Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.SUBUNIT STRUCTURE: Homodimer. Interacts with TBP. Interacts with RASL11A, TAF1A and TAF1D. Binds to IRS1 and PIK3CA By similarity. SUBCELLULAR LOCATION: Nucleus › nucleolus By similarity. PTM: Phosphorylated and activated by PIK3CA By similarity. SEQUENCE SIMILARITIES: Contains 6 HMG box DNA-binding domains. View All» Product Name: Anti-UBF, clone EP2741Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-132929.htm
        © Merck Millipore代理MABE195 Anti-UBF, clone EP2741Y Rabbit Monoclonal，产品报价联系微信jinshanbio