货号:MABE50
品牌:Merck Millipore
规格:100Ug
目录价:¥5053.00
市场价格:¥4295.05
会员价格:¥4042.40
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Description:
Anti-Phosphoepitope SR proteins Antibody, clone 1H4 | MABE50
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Molecular Weight:
~ 75, 55, 40, 30, and 20 kDa observed. This antibody recognizes the family of SR proteins which consist of 75, 55, 40, 30, 20 kDa proteins.
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Immunogen:
Purified oocyte nuclei containing xenopus SR proteins.
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Clone:
1H4
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Isotype:
IgG1κ
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Background Information:
Ser-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including; the identification and appropriation of splice-sites, and the manipulation of alternative splicing regulation.
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Species Reactivity:
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Rat
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Mouse
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Human
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Species Reactivity Note:
Demonstrated to react with rat. Predicted to react with mouse and human based on 100% sequence homology.
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Application Notes:
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC (Fukuhara, T., et al. (2006). Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Buratti, E., et al. (2007).
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Control:
L6 plus insulin cell lysate
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Quality Assurance:
Evaluated by Western Blot in L6 plus insulin cell lysate.Western Blot Analysis: 0.025 µg/mL of this antibody detected SR proteins in 10 µg of L6 plus insulin cell lysate.
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Purification Method:
Protein G Purified
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Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
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Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
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Gene Symbol:
SR proteins
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Alternate Names:
Ser-Arg-rich proteins
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Western Blotting
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Immunocytochemistry
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Immunoprecipitation
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Product Name:
Anti-Phosphoepitope SR proteins, clone 1H4
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Concentration:
0.5 mg/mL
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Antibody Type:
Monoclonal Antibody
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Qty/Pk:
100 μg
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Format:
Purified
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Host:
Mouse
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