货号：MABE57

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-E2F-6 Antibody, clone LL6-2 | MABE57 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes E2F-6. View All» Molecular Weight: ~32 kDa Observed View All» Epitope: Unknown View All» Immunogen: Histidine-tagged recombinant protein corresponding to human E2F-6. View All» Clone: LL6-2 View All» Isotype: IgG2bκ View All» Background Information: The E2F family of transcription factors includes the E2Fs and DP1. These proteins are critical regulators of genes required for cell cycle progression, and their activity is regulated by association with different protein partners during stages of the cell cycle. E2Fs bind the hypophosphorylated form of pRb, and are maintained in an inactive state. Phosphorylation of Rb causes dissociation, and activation of E2F activity. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. View All» Application Notes: Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect E2F-6 in mouse embryonic stem cells. (Courel, M., et al. (2008). Developmental Dynamics. 237:1232–1242.)Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Trimarchi, J., et al. (2001). PNAS. 98(4):1519–1524.) View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: 0.5 µg/mL of this antibody detected E2F-6 on 10 µg of HeLa cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O75461 View All» Entrez Gene Number: NP_937987 View All» Gene Symbol:
  • E2F-6

  • E2F6
    View All» Alternate Names:
    • E2F transcription factor 6

    • E2F transcription factor 6, isoform 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation
        View All» Entrez Gene Summary: This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.SUBUNIT STRUCTURE: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10.SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.SEQUENCE SIMILARITIES: Belongs to the E2F/DP family. View All» Product Name: Anti-E2F-6, clone LL6-2 Antibody View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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