货号：MABF21

品牌：Merck Millipore

规格：EA

目录价：￥3190.00

市场价格：￥2711.50

会员价格：￥2552.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-IRF-3 (N-term) Antibody, clone EPR2418Y, Rabbit | MABF21 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes IRF-3 near the N-terminus. View All» Molecular Weight: ~51 kDa observed View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to human IRF-3 near the N-terminus. View All» Clone: EPR2418Y View All» Isotype: IgG View All» Background Information: A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3). View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Proven to react with human and mouse. Species cross-reactivity is based on WB analysis. View All» Application Notes: Immunohistochemistry Analysis: A 1:100 – 1:250 dilution from a previous lot detected IRF-3 in human tonsil tissue.Flow Cytometry Analysis: A 1:20 dilution from a previous lot detected IRF-3 in FC. View All» Control: U937, 293T, HeLa, and MCF-7 cell lysates View All» Quality Assurance: Evaluated by Western Blot in U937, 293T, HeLa, and MCF-7 cell lysates.Western Blot Analysis: A 1:2,000 – 1:10,000 dilution of this antibody detected IRF-3 in U937, 293T, HeLa, and MCF-7 cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q14653 View All» Entrez Gene Number: NP_001562.1 View All» Gene Symbol: IRF-3 View All» Alternate Names: interferon regulatory factor 3 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Western Blotting

  • Immunohistochemistry (Paraffin)

  • Flow Cytometry
    View All» Entrez Gene Summary: IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants have been described. View All» UniProt Summary: FUNCTION: Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.SUBUNIT STRUCTURE: Homodimer; phosphorylation-induced. Interacts with CREBBP. May interact with MAVS. Interacts with IKBKE and TBK1. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1 (via C-terminus); this interaction leads to the proteasome-dependent degradation of IRF3. Interacts with RBCK1. Interacts with TRIM21. Interacts with HERC5. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm. TISSUE SPECIFICITY: Expressed constitutively in a variety of tissues.PTM: Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3. Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation. ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. SEQUENCE SIMILARITIES: Belongs to the IRF family.Contains 1 IRF tryptophan pentad repeat DNA-binding domain. View All» Product Name: Anti-IRF-3 (N-term), clone EPR2418Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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